PMID- 20418432
OWN - NLM
STAT- MEDLINE
DCOM- 20100928
LR  - 20211020
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 76
IP  - 12
DP  - 2010 Jun
TI  - Analysis of the fine-scale population structure of "Candidatus accumulibacter 
      phosphatis" in enhanced biological phosphorus removal sludge, using fluorescence 
      in situ hybridization and flow cytometric sorting.
PG  - 3825-35
LID - 10.1128/AEM.00260-10 [doi]
AB  - To investigate the fine-scale diversity of the polyphosphate-accumulating 
      organisms (PAO) "Candidatus Accumulibacter phosphatis" (henceforth referred to as 
      "Ca. Accumulibacter"), two laboratory-scale sequencing batch reactors (SBRs) for 
      enhanced biological phosphorus removal (EBPR) were operated with sodium acetate 
      as the sole carbon source. During SBR operations, activated sludge always 
      contained morphologically different "Ca. Accumulibacter" strains showing typical 
      EBPR performances, as confirmed by the combined technique of fluorescence in situ 
      hybridization (FISH) and microautoradiography (MAR). Fragments of "Ca. 
      Accumulibacter" 16S rRNA genes were retrieved from the sludge. Phylogenetic 
      analyses together with sequences from the GenBank database showed that "Ca. 
      Accumulibacter" 16S rRNA genes of the EBPR sludge were clearly differentiated 
      into four "Ca. Accumulibacter" clades, Acc-SG1, Acc-SG2, Acc-SG3, and Acc-SG4. 
      The specific FISH probes Acc444, Acc184, Acc72, and Acc119 targeting these clades 
      and some helpers and competitors were designed by using the ARB program. 
      Microbial characterization by FISH analysis using specific FISH probes also 
      clearly indicated the presence of different "Ca. Accumulibacter" cell 
      morphotypes. Especially, members of Acc-SG3, targeted by probe Acc72, were 
      coccobacillus-shaped cells with a size of approximately 2 to 3 mum, while members 
      of Acc-SG1, Acc-SG2, and Acc-SG4, targeted by Acc444, Acc184, and Acc119, 
      respectively, were coccus-shaped cells approximately 1 mum in size. Subsequently, 
      cells targeted by each FISH probe were sorted by use of a flow cytometer, and 
      their polyphosphate kinase 1 (ppk1) gene homologs were amplified by using a 
      ppk1-specific PCR primer set for "Ca. Accumulibacter." The phylogenetic tree 
      based on sequences of the ppk1 gene homologs was basically congruent with that of 
      the 16S rRNA genes, but members of Acc-SG3 with a distinct morphology comprised 
      two different ppk1 genes. These results suggest that "Ca. Accumulibacter" strains 
      may be diverse physiologically and ecologically and represent distinct 
      populations with genetically determined adaptations in EBPR systems.
FAU - Kim, Jeong Myeong
AU  - Kim JM
AD  - Department of Life Science, Chung-Ang University, 221 HeukSeok-Dong, Seoul 
      156-756, Republic of Korea.
FAU - Lee, Hyo Jung
AU  - Lee HJ
FAU - Kim, Sun Young
AU  - Kim SY
FAU - Song, Jae Jun
AU  - Song JJ
FAU - Park, Woojun
AU  - Park W
FAU - Jeon, Che Ok
AU  - Jeon CO
LA  - eng
SI  - GENBANK/HM046414
SI  - GENBANK/HM046415
SI  - GENBANK/HM046416
SI  - GENBANK/HM046417
SI  - GENBANK/HM046418
SI  - GENBANK/HM046419
SI  - GENBANK/HM046420
SI  - GENBANK/HM046421
SI  - GENBANK/HM046422
SI  - GENBANK/HM046423
SI  - GENBANK/HM046424
SI  - GENBANK/HM046425
SI  - GENBANK/HM046426
SI  - GENBANK/HM046427
SI  - GENBANK/HM046428
SI  - GENBANK/HM046429
SI  - GENBANK/HM046430
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100423
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
RN  - 4550K0SC9B (Sodium Acetate)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Betaproteobacteria/*classification/genetics/*isolation & purification/metabolism
MH  - Bioreactors/microbiology
MH  - Carbon/metabolism
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Flow Cytometry/methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Metagenome
MH  - Molecular Sequence Data
MH  - Phosphorus/*metabolism
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - Sodium Acetate/metabolism
PMC - PMC2893498
EDAT- 2010/04/27 06:00
MHDA- 2010/09/30 06:00
PMCR- 2010/12/01
CRDT- 2010/04/27 06:00
PHST- 2010/04/27 06:00 [entrez]
PHST- 2010/04/27 06:00 [pubmed]
PHST- 2010/09/30 06:00 [medline]
PHST- 2010/12/01 00:00 [pmc-release]
AID - AEM.00260-10 [pii]
AID - 0260-10 [pii]
AID - 10.1128/AEM.00260-10 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2010 Jun;76(12):3825-35. doi: 10.1128/AEM.00260-10. Epub 
      2010 Apr 23.