PMID- 20419821
OWN - NLM
STAT- MEDLINE
DCOM- 20100719
LR  - 20211020
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Linking)
VI  - 82
IP  - 6
DP  - 2010 May
TI  - Detection of viral DNA sequences in the cerebrospinal fluid of patients with 
      multiple sclerosis.
PG  - 1051-7
LID - 10.1002/jmv.21764 [doi]
AB  - The role of viruses in the pathogenesis of multiple sclerosis (MS) is a subject 
      of heated debate. The presence of six different neurotropic viruses was sought, 
      including JC virus (JCV), varicella zoster virus (VZV), human herpesvirus 6 
      (HHV-6), and Epstein-Barr virus (EBV), in cerebrospinal fluid (CSF) samples 
      collected from 51 patients with MS and 30 patients with other neurological 
      diseases. Cell-free or cell-associated viral DNA in CSF samples was detected by 
      real-time PCR, and viral loads were determined. Magnetic resonance imaging (MRI) 
      examinations were also performed to look for active lesions. Cell-associated JCV 
      DNA was detected in 3 of the 51 patients with MS and in 2 of the 30 patients with 
      other neurological disease. Cell-free JCV DNA was detected in one additional 
      patient with MS. Cell-free VZV DNA was detected in one patient without MS, 
      cell-free HHV-6 was detected in one patient with MS, and cell-free EBV was 
      detected in one patient with MS. All other study patients had no detectable viral 
      DNA in CSF samples and no double infections were found. The small percentage of 
      patients with detectable viral DNA in CSF samples was comparable between patients 
      with MS and those with other neurological disease, and presence of viral DNA was 
      not a predictor of brain lesions. Additional observations suggest that cell 
      trafficking from the periphery, rather than leakage through the blood-brain 
      barrier, results in the transport of viruses to the CNS, where local 
      immunosurveillance can control viral replication in immunocompetent individuals.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Mancuso, Roberta
AU  - Mancuso R
AD  - Laboratory of Molecular Medicine and Biotechnology, Don C Gnocchi Foundation, 
      IRCCS S Maria Nascente, Milan, Italy. rmancuso@dongnocchi.it
FAU - Hernis, Ambra
AU  - Hernis A
FAU - Cavarretta, Rosella
AU  - Cavarretta R
FAU - Caputo, Domenico
AU  - Caputo D
FAU - Calabrese, Elena
AU  - Calabrese E
FAU - Nemni, Raffaello
AU  - Nemni R
FAU - Ferrante, Pasquale
AU  - Ferrante P
FAU - Delbue, Serena
AU  - Delbue S
FAU - Clerici, Mario
AU  - Clerici M
LA  - eng
GR  - R01 MH072528/MH/NIMH NIH HHS/United States
GR  - R01 MH072528-06/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cerebrospinal Fluid/*virology
MH  - DNA, Viral/*genetics/*isolation & purification
MH  - Female
MH  - Herpesvirus 3, Human/genetics
MH  - Herpesvirus 4, Human/genetics
MH  - Herpesvirus 6, Human/genetics
MH  - Humans
MH  - JC Virus/genetics
MH  - Male
MH  - Middle Aged
MH  - Multiple Sclerosis/*virology
MH  - Young Adult
EDAT- 2010/04/27 06:00
MHDA- 2010/07/20 06:00
CRDT- 2010/04/27 06:00
PHST- 2010/04/27 06:00 [entrez]
PHST- 2010/04/27 06:00 [pubmed]
PHST- 2010/07/20 06:00 [medline]
AID - 10.1002/jmv.21764 [doi]
PST - ppublish
SO  - J Med Virol. 2010 May;82(6):1051-7. doi: 10.1002/jmv.21764.