PMID- 20420024
OWN - NLM
STAT- MEDLINE
DCOM- 20100514
LR  - 20100427
IS  - 1015-8146 (Print)
IS  - 1015-8146 (Linking)
VI  - 21
IP  - 1
DP  - 2010
TI  - SRY gene amplifications and genotypings revealed the occurrence of the hidden 
      maternal decidual cells in 46,XX karyotyped spontaneous abortions.
PG  - 9-17
AB  - Reports of abnormal karyotypes or normal 46,XY karyotypes of the abortion 
      materials derivated from tissue cultures are mostly addressing the pregnancy loss 
      tissues. The accuracy of the cytogenetic reports of normal 46,XX karyotypes is 
      obscure, as the results may reflect the normal karyotyped female pregnancy losses 
      or the hidden maternal decidual cells covering the cytogenetically normal or 
      abnormal male or female products of conception. In the present study, 
      thirty-eight 46,XX normal karyotyped abortion materials cultivated from villi 
      were re-analysed for excluding maternal cell contamination by using molecular 
      approaches in an accurate algorithm. Abortion materials DNAs were amplified by 
      polymerase chain reaction (PCR) technique in order to search the products of the 
      sex determinating region gene of chromosome Y (SRY). Sixteen out of 38 abortion 
      materials revealed Y-chromosome component (42.1%). Amplification negative DNAs 
      and their parental DNAs were genotyped by using high-polymorphic microsatellite 
      DNA markers to identify the origin of the components of the chromosome X. 
      Maternal chromosome X components were detected in 18 (81.8%). As a result, SRY 
      amplifications and genotypings ascertained the high rate of maternal decidual 
      cells in 46,XX products of conception.
FAU - Karaoguz, M Y
AU  - Karaoguz MY
AD  - Department of Medical Genetics, Gazi University, Faculty of Medicine, Ankara, 
      Turkey. karaoguz@gazi.edu.tr
FAU - Percin, E F
AU  - Percin EF
FAU - Pala, E
AU  - Pala E
FAU - Biri, A A
AU  - Biri AA
FAU - Korucuoglu, U
AU  - Korucuoglu U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Genet Couns
JT  - Genetic counseling (Geneva, Switzerland)
JID - 9015261
SB  - IM
MH  - Abortion, Spontaneous/*genetics
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Cytogenetic Analysis/*methods
MH  - Decidua/*cytology
MH  - Diagnostic Errors
MH  - Female
MH  - Genes, sry/*genetics
MH  - Humans
MH  - Karyotyping
MH  - Polymerase Chain Reaction
EDAT- 2010/04/28 06:00
MHDA- 2010/05/15 06:00
CRDT- 2010/04/28 06:00
PHST- 2010/04/28 06:00 [entrez]
PHST- 2010/04/28 06:00 [pubmed]
PHST- 2010/05/15 06:00 [medline]
PST - ppublish
SO  - Genet Couns. 2010;21(1):9-17.