PMID- 20420816
OWN - NLM
STAT- MEDLINE
DCOM- 20101019
LR  - 20210224
IS  - 1873-2941 (Electronic)
IS  - 0009-3084 (Linking)
VI  - 163
IP  - 6
DP  - 2010 Jun
TI  - Significant sensitivity improvements by matrix optimization: a MALDI-TOF mass 
      spectrometric study of lipids from hen egg yolk.
PG  - 552-60
LID - 10.1016/j.chemphyslip.2010.04.005 [doi]
AB  - Due to its sensitivity, the tolerance of impurities and the simplicity of 
      performance, matrix-assisted laser desorption and ionization time-of-flight 
      (MALDI-TOF) mass spectrometry (MS) is increasingly used to analyze lipids from 
      biological sources. Although its detailed role is not understood so far, the 
      applied matrix has a pronounced effect on the achievable spectrum quality and 
      particularly how sensitive the individual lipid classes are detectable. Different 
      matrix compounds were recently established in the lipid field including 
      2,5-dihydroxybenzoic acid (DHB), 9-aminoacridine (9-AA), para-nitroaniline (PNA), 
      2-mercaptobenzothiazole (MBT), and 2-(2-aminoethylamino)-5-nitropyridine (AAN). 
      It is the aim of this paper to compare the properties of these matrices with the 
      newly synthesized matrix, alpha-cyano-2,4-difluorocinnamic acid (Di-FCCA). An 
      organic extract from hen egg yolk was used as a simple and easily available test 
      system. It will be shown that Di-FCCA is the matrix of choice to detect lipids in 
      the positive-ion mode due to an achievable sensitivity gain of more than one 
      order of magnitude compared to alternative matrices. In contrast, Di-FCCA is not 
      suitable for negative-ion detection of phospholipids. Here, 9-AA is unequivocally 
      the matrix of choice.
CI  - Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
FAU - Teuber, Kristin
AU  - Teuber K
AD  - LIFE Center, University of Leipzig, Germany.
FAU - Schiller, Jurgen
AU  - Schiller J
FAU - Fuchs, Beate
AU  - Fuchs B
FAU - Karas, Michael
AU  - Karas M
FAU - Jaskolla, Thorsten W
AU  - Jaskolla TW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100424
PL  - Ireland
TA  - Chem Phys Lipids
JT  - Chemistry and physics of lipids
JID - 0067206
RN  - 0 (2-(2-aminoethylamino)-5-nitropyridine)
RN  - 0 (Aniline Compounds)
RN  - 0 (Benzothiazoles)
RN  - 0 (Cinnamates)
RN  - 0 (Gentisates)
RN  - 0 (Lipids)
RN  - 0 (Pyridines)
RN  - 140-10-3 (cinnamic acid)
RN  - 1MRQ0QZG7G (4-nitroaniline)
RN  - 5RLR54Z22K (captax)
RN  - 78OY3Z0P7Z (Aminacrine)
RN  - VP36V95O3T (2,5-dihydroxybenzoic acid)
SB  - IM
MH  - Aminacrine/analysis
MH  - Aniline Compounds/analysis
MH  - Animals
MH  - Benzothiazoles/analysis
MH  - Chickens
MH  - Cinnamates/chemistry
MH  - Egg Yolk/*chemistry
MH  - Gentisates/analysis
MH  - Lipids/*analysis
MH  - Pyridines/analysis
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
EDAT- 2010/04/28 06:00
MHDA- 2010/10/20 06:00
CRDT- 2010/04/28 06:00
PHST- 2009/12/03 00:00 [received]
PHST- 2010/04/07 00:00 [revised]
PHST- 2010/04/15 00:00 [accepted]
PHST- 2010/04/28 06:00 [entrez]
PHST- 2010/04/28 06:00 [pubmed]
PHST- 2010/10/20 06:00 [medline]
AID - S0009-3084(10)00071-X [pii]
AID - 10.1016/j.chemphyslip.2010.04.005 [doi]
PST - ppublish
SO  - Chem Phys Lipids. 2010 Jun;163(6):552-60. doi: 10.1016/j.chemphyslip.2010.04.005. 
      Epub 2010 Apr 24.