PMID- 20421778
OWN - NLM
STAT- MEDLINE
DCOM- 20100615
LR  - 20220208
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 34
IP  - 6
DP  - 2010 Jun
TI  - Dual-color, break-apart FISH assay on paraffin-embedded tissues as an adjunct to 
      diagnosis of Xp11 translocation renal cell carcinoma and alveolar soft part 
      sarcoma.
PG  - 757-66
LID - 10.1097/PAS.0b013e3181dd577e [doi]
AB  - Both Xp11.2 translocation renal cell carcinoma (RCC) and alveolar soft part 
      sarcoma (ASPS) are characterized by various translocations disrupting chromosome 
      Xp11.2, which result in gene fusions involving the TFE3 transcription factor 
      gene. Diagnostic tools to detect translocations involving the TFE3 gene on 
      chromosome X would be valuable in the evaluation of these tumors. We developed a 
      dual-color, break-apart fluorescence in situ hybridization (FISH) assay to 
      identify the chromosomal break point in paraffin-embedded tissue. This assay was 
      validated using 4 cases of Xp11.2 RCC [proven by karyotype and/or 
      reverse-transcriptase polymerase chain reaction (RT-PCR)], 2 cases of ASPS 
      (proven by karyotype or RT-PCR), the UOK109 cell line carrying the inv(X) 
      (p11;q12), and several negative controls (both neoplastic and non-neoplastic). 
      This break-apart FISH assay is a relatively quick procedure for detecting Xp11.2 
      RCC and ASPS translocations and can be applied to archival paraffin-embedded 
      tissue.
FAU - Zhong, Minghao
AU  - Zhong M
AD  - Pathology and Laboratory Medicine, University of Medicine and Dentistry of New 
      Jersey-New Jersey Medical School, Newark, NJ, USA. Hameedm@mskcc.org
FAU - De Angelo, Patricia
AU  - De Angelo P
FAU - Osborne, Lisa
AU  - Osborne L
FAU - Keane-Tarchichi, Megan
AU  - Keane-Tarchichi M
FAU - Goldfischer, Michael
AU  - Goldfischer M
FAU - Edelmann, Lisa
AU  - Edelmann L
FAU - Yang, Youfeng
AU  - Yang Y
FAU - Linehan, W Marston
AU  - Linehan WM
FAU - Merino, Maria J
AU  - Merino MJ
FAU - Aisner, Seena
AU  - Aisner S
FAU - Hameed, Meera
AU  - Hameed M
LA  - eng
GR  - Z01 BC010696/ImNIH/Intramural NIH HHS/United States
GR  - Z01 SC006659/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)
RN  - 0 (TFE3 protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics
MH  - Carcinoma, Renal Cell/diagnosis/*genetics
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, X/*genetics
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Kidney Neoplasms/diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Paraffin Embedding
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sarcoma, Alveolar Soft Part/diagnosis/*genetics
MH  - Sensitivity and Specificity
MH  - Translocation, Genetic
PMC - PMC7386799
MID - NIHMS1602313
EDAT- 2010/04/28 06:00
MHDA- 2010/06/16 06:00
PMCR- 2020/07/28
CRDT- 2010/04/28 06:00
PHST- 2010/04/28 06:00 [entrez]
PHST- 2010/04/28 06:00 [pubmed]
PHST- 2010/06/16 06:00 [medline]
PHST- 2020/07/28 00:00 [pmc-release]
AID - 10.1097/PAS.0b013e3181dd577e [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2010 Jun;34(6):757-66. doi: 10.1097/PAS.0b013e3181dd577e.