PMID- 20423521
OWN - NLM
STAT- MEDLINE
DCOM- 20100715
LR  - 20211020
IS  - 1471-2334 (Electronic)
IS  - 1471-2334 (Linking)
VI  - 10
DP  - 2010 Apr 28
TI  - Cellular immunity in children with successful immunoprophylactic treatment for 
      mother-to-child transmission of hepatitis B virus.
PG  - 103
LID - 10.1186/1471-2334-10-103 [doi]
AB  - BACKGROUND: The administration of hepatitis B immunoglobulin followed by 
      hepatitis B vaccine can result in a protective efficacy of almost 90% in 
      mother-to-child transmission of hepatitis B virus (HBV). However, little is known 
      about immunity against HBV infection in children after immunoprophylactic 
      treatment. We tried to assess the association between T-cell responses and 
      viremia in children after successful prophylactic treatment. METHODS: Thirteen 
      children and their 8 HBV carrier mothers (8 families), who were positive for 
      human leukocyte antigen (HLA)-A24, were enrolled in this study. All of the 13 
      children received immunoprophylactic treatment and became negative for hepatitis 
      B surface antigen (HBsAg) after birth. HBV-specific cytotoxic T lymphocyte (CTL) 
      responses were evaluated using IFNgamma - enzyme-linked immunosorbent spot 
      (ELISPOT) and major histocompatibility complex class I peptide pentamer assays. 
      Serum HBV DNA was measured by real-time PCR. RESULTS: Significant HBV-specific 
      T-cell responses were detected in 2 (15%) of the 13 children by ELISPOT. However, 
      the frequency of HLA-A24-HBV-specific CTLs was very low in both HBV carrier 
      mothers and children using pentamers. Of the 13 children, 4 (31%) were positive 
      for serum HBV DNA. However, the levels of serum HBV DNA were 100 copies/ml or 
      less. One of the 2 children in whom significant HBV-specific CTL responses were 
      detectable was positive for serum HBV DNA. CONCLUSIONS: HBV core and 
      polymerase-specific T-cell responses were detected and a low-dose viremia was 
      observed in children after successful immunoprophylaxis treatment. Although the 
      presence of viremia was not related to HBV-specific T-cell responses, CTLs might 
      play a role in the control of HBV infection in children born to HBsAg-positive 
      mothers after immunoprophylactic treatment.
FAU - Komatsu, Haruki
AU  - Komatsu H
AD  - Department of Pediatrics, Yokohama Eastern Hospital, 3-6-1 Shimosueyoshi Tsurumi 
      Yokohama, Kanagawa 230-0012, Japan. haruki-komatsu@chive.ocn.ne.jp
FAU - Inui, Ayano
AU  - Inui A
FAU - Sogo, Tsuyoshi
AU  - Sogo T
FAU - Hiejima, Eitaro
AU  - Hiejima E
FAU - Tateno, Akihiko
AU  - Tateno A
FAU - Klenerman, Paul
AU  - Klenerman P
FAU - Fujisawa, Tomoo
AU  - Fujisawa T
LA  - eng
PT  - Journal Article
DEP - 20100428
PL  - England
TA  - BMC Infect Dis
JT  - BMC infectious diseases
JID - 100968551
RN  - 0 (DNA, Viral)
RN  - 0 (Hepatitis B Antibodies)
RN  - 0 (Hepatitis B Vaccines)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral/blood
MH  - Female
MH  - Hepatitis B/*immunology/*prevention & control
MH  - Hepatitis B Antibodies/*therapeutic use
MH  - Hepatitis B Vaccines/*administration & dosage
MH  - Hepatitis B virus/*immunology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - *Immunity, Cellular
MH  - Infant
MH  - Infant, Newborn
MH  - Infectious Disease Transmission, Vertical/*prevention & control
MH  - Interferon-gamma/metabolism
MH  - Male
MH  - Middle Aged
MH  - Serum/virology
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Viremia
MH  - Young Adult
PMC - PMC2879245
EDAT- 2010/04/29 06:00
MHDA- 2010/07/16 06:00
PMCR- 2010/04/28
CRDT- 2010/04/29 06:00
PHST- 2010/01/01 00:00 [received]
PHST- 2010/04/28 00:00 [accepted]
PHST- 2010/04/29 06:00 [entrez]
PHST- 2010/04/29 06:00 [pubmed]
PHST- 2010/07/16 06:00 [medline]
PHST- 2010/04/28 00:00 [pmc-release]
AID - 1471-2334-10-103 [pii]
AID - 10.1186/1471-2334-10-103 [doi]
PST - epublish
SO  - BMC Infect Dis. 2010 Apr 28;10:103. doi: 10.1186/1471-2334-10-103.