PMID- 20424918
OWN - NLM
STAT- MEDLINE
DCOM- 20110321
LR  - 20220317
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 38
IP  - 1
DP  - 2011 Jan
TI  - Association of polymorphisms in the human IL-10 and IL-18 genes with rheumatoid 
      arthritis.
PG  - 379-85
LID - 10.1007/s11033-010-0119-x [doi]
AB  - The decrease of anti-inflammatory cytokine and increase of pro-inflammatory 
      cytokine was observed in rheumatoid arthritis (RA). Interleukin-10 (IL-10), a 
      potent anti-inflammatory cytokine, has been demonstrated to suppress joint 
      swelling and deformation in RA animal model. Interleukin-18 (IL-18), a widely 
      distributed pro-inflammatory cytokine, induces the production of IFN-gamma, activate 
      NK cells, and promote inflammation. Recent studies demonstrated that the serum 
      IL-10 and IL-18 levels may be influenced by genetics and related to 
      susceptibility to several autoimmune diseases. In the present study, using 
      polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and 
      DNA sequencing techniques, we analyzed the genotype and allele distributions of 
      two single nucleotide polymorphisms (SNP) loci in the promoter region of IL-10 
      and IL-18 genes (IL-10-592 A/C and IL-18-607 A/C loci, respectively). Our results 
      indicated that IL-10-592 allelic and genotypic frequencies were significantly 
      different between the RA patients and normal subjects (P<0.05). In addition, 
      significant differences of IL-10-592 allelic and genotypic frequencies were also 
      detected between the patients with or without anti-cyclic citrullinated peptide 
      antibody (anti-CCP) (P<0.05). In contrast, allelic and genotypic frequencies of 
      IL-18-607 did not show significant difference between RA patients and normal 
      subjects (P>0.05) or between anti-CCP-positive and anti-CCP-negative RA patients 
      (P>0.05). Furthermore, ELISA detection of IL-10 and IL-18 serum levels revealed 
      that the genotype of IL-10-592 was associated with IL-10 serum level (P<0.05), 
      but the genotype and allele frequency of IL-18-607 was not associated with IL-18 
      serum level (P>0.05). Taken together, our findings provide new insight for the 
      polymorphism of IL-10 gene in the pathogenesis of RA.
FAU - Ying, Binwu
AU  - Ying B
AD  - Department of Laboratory Medicine, West China Hospital, Sichuan University, 
      Chengdu, 610041, Sichuan Province, People's Republic of China.
FAU - Shi, Yunying
AU  - Shi Y
FAU - Pan, Xiaofu
AU  - Pan X
FAU - Song, Xingbo
AU  - Song X
FAU - Huang, Zhunchun
AU  - Huang Z
FAU - Niu, Qian
AU  - Niu Q
FAU - Cai, Bei
AU  - Cai B
FAU - Wang, Lanlan
AU  - Wang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100428
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Antibodies)
RN  - 0 (IL10 protein, human)
RN  - 0 (Interleukin-18)
RN  - 0 (Peptides, Cyclic)
RN  - 0 (cyclic citrullinated peptide)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adult
MH  - Antibodies/immunology
MH  - Arthritis, Rheumatoid/blood/*genetics/immunology
MH  - Case-Control Studies
MH  - Female
MH  - *Genetic Association Studies
MH  - Genetic Loci/genetics
MH  - *Genetic Predisposition to Disease
MH  - Humans
MH  - Interleukin-10/blood/*genetics
MH  - Interleukin-18/blood/*genetics
MH  - Male
MH  - Peptides, Cyclic/immunology
MH  - Pilot Projects
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Titrimetry
EDAT- 2010/04/29 06:00
MHDA- 2011/03/22 06:00
CRDT- 2010/04/29 06:00
PHST- 2009/11/04 00:00 [received]
PHST- 2010/03/17 00:00 [accepted]
PHST- 2010/04/29 06:00 [entrez]
PHST- 2010/04/29 06:00 [pubmed]
PHST- 2011/03/22 06:00 [medline]
AID - 10.1007/s11033-010-0119-x [doi]
PST - ppublish
SO  - Mol Biol Rep. 2011 Jan;38(1):379-85. doi: 10.1007/s11033-010-0119-x. Epub 2010 
      Apr 28.