PMID- 20426746
OWN - NLM
STAT- MEDLINE
DCOM- 20110202
LR  - 20190923
IS  - 1875-5402 (Electronic)
IS  - 1386-2073 (Linking)
VI  - 13
IP  - 7
DP  - 2010 Aug
TI  - Development of a label free IGE sensitive aptasensor based on electrochemical 
      impedance spectrometry.
PG  - 578-81
AB  - A sensitive aptamer based electrochemical biosensor to detect human 
      immunoglobulin E (IgE) is presented in this study. 5' Biotin labeled 45 mer DNA 
      aptamer sequence was immobilized onto streptavidin coated graphite surfaces. 
      Interaction between human IgE and DNA aptamer was monitored by Electrochemical 
      Impedance Spectrometry (EIS) in a 0.48nM detection limit of IgE. EIS analysis are 
      based on electron transfer resistance (Rct) in the presence of 5mM 
      [Fe(CN)6]3-/4-.
FAU - Kara, Pinar
AU  - Kara P
AD  - Department of Analytical Chemistry, Ege University, Bornova, Izmir, Turkey. 
      pinar.kara@ege.edu.tr
FAU - Meric, Buket
AU  - Meric B
FAU - Ozsoz, Mehmet
AU  - Ozsoz M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United Arab Emirates
TA  - Comb Chem High Throughput Screen
JT  - Combinatorial chemistry & high throughput screening
JID - 9810948
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7782-42-5 (Graphite)
RN  - 9013-20-1 (Streptavidin)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biosensing Techniques/instrumentation/*methods
MH  - Coated Materials, Biocompatible/chemistry
MH  - Electric Impedance
MH  - Electrochemistry
MH  - Graphite/chemistry
MH  - High-Throughput Screening Assays/instrumentation/methods
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Sensitivity and Specificity
MH  - Streptavidin/chemistry
MH  - Surface Properties
EDAT- 2010/04/30 06:00
MHDA- 2011/02/03 06:00
CRDT- 2010/04/30 06:00
PHST- 2009/12/29 00:00 [received]
PHST- 2010/01/25 00:00 [accepted]
PHST- 2010/04/30 06:00 [entrez]
PHST- 2010/04/30 06:00 [pubmed]
PHST- 2011/02/03 06:00 [medline]
AID - BSP/CCHTS/E-Pub/00085 [pii]
AID - 10.2174/1386207311004070578 [doi]
PST - ppublish
SO  - Comb Chem High Throughput Screen. 2010 Aug;13(7):578-81. doi: 
      10.2174/1386207311004070578.