PMID- 20426769
OWN - NLM
STAT- MEDLINE
DCOM- 20100720
LR  - 20121115
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 107
IP  - 6
DP  - 2009 Dec 1
TI  - Comparison of DNA- and RNA-based bacterial community structures in soil exposed 
      to 2,4-dichlorophenol.
PG  - 1883-93
LID - 10.1111/j.1365-2672.2009.04369.x [doi]
AB  - AIMS: To examine the effect of the pollutant 2,4-dichlorophenol on DNA- and 
      RNA-based bacterial communities in soil. METHODS AND RESULTS: Soil was exposed to 
      100 mg kg(-1) of 2,4-dichlorophenol (2,4-DCP), and degradation was monitored over 
      35 days. DNA and RNA were coextracted, and terminal restriction fragment length 
      polymorphism (T-RFLP) was used to report changes in bacterial communities in 
      response to the presence of the chlorophenol. The phylogenetic composition of the 
      soil during degradation was determined by creating a clone library of amplified 
      16S rRNA sequences from both DNA and reverse-transcribed RNA from exposed soil. 
      Resulting clones were sequenced, and putative identities were assigned. 
      CONCLUSIONS: A significant difference between active (RNA-based) and total 
      (DNA-based) bacterial community structure was observed for both T-RFLP and 
      phylogenetic analyses in response to 2,4-DCP, with more pronounced changes seen 
      in RNA-based communities. Phylogenetic analysis indicated the dominance of 
      Proteobacteria in both profiles. SIGNIFICANCE AND IMPACT OF THE STUDY: This study 
      describes the response of soil bacterial communities to the addition of the 
      xenobiotic compound 2,4-DCP, and highlights the importance of including RNA-based 
      16S rRNA analysis to complement any molecular study in a perturbed soil.
FAU - Lillis, L
AU  - Lillis L
AD  - School of Biology and Environmental Science, University College Dublin, Belfield, 
      Dublin, Ireland. lorraine.lillis@ucd.ie
FAU - Doyle, E
AU  - Doyle E
FAU - Clipson, N
AU  - Clipson N
LA  - eng
SI  - GENBANK/EU247456
SI  - GENBANK/EU247457
SI  - GENBANK/EU247458
SI  - GENBANK/EU247459
SI  - GENBANK/EU247460
SI  - GENBANK/EU247461
SI  - GENBANK/EU247462
SI  - GENBANK/EU247463
SI  - GENBANK/EU247464
SI  - GENBANK/EU247465
SI  - GENBANK/EU247466
SI  - GENBANK/EU247467
SI  - GENBANK/EU247468
SI  - GENBANK/EU247469
SI  - GENBANK/EU247470
SI  - GENBANK/EU247471
SI  - GENBANK/EU247472
SI  - GENBANK/EU247473
SI  - GENBANK/EU247474
SI  - GENBANK/EU247475
SI  - GENBANK/EU247476
SI  - GENBANK/EU247477
SI  - GENBANK/EU247478
SI  - GENBANK/EU247479
SI  - GENBANK/EU247480
SI  - GENBANK/EU247481
SI  - GENBANK/EU247482
SI  - GENBANK/EU247483
SI  - GENBANK/EU247484
SI  - GENBANK/EU247485
SI  - GENBANK/EU247486
SI  - GENBANK/EU247487
SI  - GENBANK/EU247488
SI  - GENBANK/EU247489
SI  - GENBANK/EU247490
SI  - GENBANK/EU247491
SI  - GENBANK/EU247492
SI  - GENBANK/EU247493
SI  - GENBANK/EU247494
SI  - GENBANK/EU247495
SI  - GENBANK/EU247496
SI  - GENBANK/EU247497
SI  - GENBANK/EU247498
SI  - GENBANK/EU247499
SI  - GENBANK/EU247500
SI  - GENBANK/EU247501
SI  - GENBANK/EU247502
SI  - GENBANK/EU247503
SI  - GENBANK/EU247504
SI  - GENBANK/EU247505
SI  - GENBANK/EU247506
SI  - GENBANK/EU247507
SI  - GENBANK/EU514547
SI  - GENBANK/EU514548
SI  - GENBANK/EU514549
SI  - GENBANK/EU514550
SI  - GENBANK/EU514551
SI  - GENBANK/EU514552
SI  - GENBANK/EU514553
SI  - GENBANK/EU514554
SI  - GENBANK/EU514555
SI  - GENBANK/EU514556
SI  - GENBANK/EU514557
SI  - GENBANK/EU514558
SI  - GENBANK/EU514559
SI  - GENBANK/EU514560
SI  - GENBANK/EU514561
SI  - GENBANK/EU514562
SI  - GENBANK/EU514564
SI  - GENBANK/EU514566
SI  - GENBANK/EU514568
SI  - GENBANK/EU514569
SI  - GENBANK/EU514570
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Chlorophenols)
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil Pollutants)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Bacteria/*classification/genetics/isolation & purification
MH  - Biodegradation, Environmental
MH  - Biodiversity
MH  - Chlorophenols/*pharmacology
MH  - DNA, Bacterial/*genetics
MH  - Metagenome/*drug effects
MH  - Molecular Sequence Data
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Bacterial/*genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Soil Microbiology
MH  - Soil Pollutants/*pharmacology
EDAT- 2010/04/30 06:00
MHDA- 2010/07/21 06:00
CRDT- 2010/04/30 06:00
PHST- 2010/04/30 06:00 [entrez]
PHST- 2010/04/30 06:00 [pubmed]
PHST- 2010/07/21 06:00 [medline]
AID - JAM4369 [pii]
AID - 10.1111/j.1365-2672.2009.04369.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2009 Dec 1;107(6):1883-93. doi: 
      10.1111/j.1365-2672.2009.04369.x.