PMID- 20427127
OWN - NLM
STAT- MEDLINE
DCOM- 20100812
LR  - 20191210
IS  - 1873-3336 (Electronic)
IS  - 0304-3894 (Linking)
VI  - 179
IP  - 1-3
DP  - 2010 Jul 15
TI  - Sensitivity of morphological change of Vero cells exposed to lipophilic compounds 
      and its mechanism.
PG  - 1055-64
LID - 10.1016/j.jhazmat.2010.03.113 [doi]
AB  - To find a sensitive cytotoxic response to reflect the toxicity of trace organic 
      pollutants, the sensitivity and reliability of morphological change and 
      proliferation inhibition of Vero cells exposed to lipophilic compounds and the 
      leachate from products related to drinking water (PRDW) were compared, and the 
      mechanism of the morphological change in Vero cells was studied. Results showed 
      the proportion of morphologically changed cells increased with increasing 
      2,4,6-trichlorophenol (TCP)/perfluorooctane sulfonate (PFOS) concentration. 
      However, at low TCP concentrations, inhibition of cell proliferation did not 
      correlate to TCP concentration. After exposure to the leachate from PRDW 
      extracted at different temperatures, the percentage of morphologically changed 
      cells increased with extracting temperature, but the inhibition of cell 
      proliferation failed to reflect the correlation to extracting temperature. These 
      imply cell morphological change is a more sensitive and reliable method to 
      reflect toxicity of trace organic pollutants than proliferation inhibition. Flow 
      cytometry analysis indicated cell membrane damage was an early and sensitive 
      cytotoxic response comparing with necrosis, resulting in cell morphological 
      change, which may be due to the interference of lipophilic compounds. Lipophilic 
      compound accumulated in cell membrane to interfere the assembly process of 
      membrane protein and phospholipid.
CI  - 2010 Elsevier B.V. All rights reserved.
FAU - Liao, Ting Ting
AU  - Liao TT
AD  - College of Environmental Science and Engineering, State Key Laboratory of 
      Pollution Control and Resource Reuse, Tongji University, Shanghai, China. 
      liaotingting 82@163.com
FAU - Shi, Yan Ling
AU  - Shi YL
FAU - Jia, Jian Wei
AU  - Jia JW
FAU - Jia, Ru Wen
AU  - Jia RW
FAU - Wang, Lei
AU  - Wang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100402
PL  - Netherlands
TA  - J Hazard Mater
JT  - Journal of hazardous materials
JID - 9422688
RN  - 0 (Chlorophenols)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Lipids)
RN  - 0 (Membrane Proteins)
RN  - 0 (Organic Chemicals)
RN  - 0 (Phospholipids)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 0 (Water Pollutants, Chemical)
RN  - 7440-44-0 (Carbon)
RN  - 9007-49-2 (DNA)
RN  - EUY85H477I (thiazolyl blue)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
SB  - IM
MH  - Animals
MH  - Carbon/analysis
MH  - Cell Membrane/drug effects/ultrastructure
MH  - Cell Proliferation/drug effects
MH  - Cell Shape/*drug effects
MH  - Chlorocebus aethiops
MH  - Chlorophenols/toxicity
MH  - DNA/analysis/biosynthesis
MH  - Environmental Pollutants/analysis/toxicity
MH  - Flow Cytometry
MH  - Lipids/chemistry/*toxicity
MH  - Membrane Proteins/chemistry/metabolism
MH  - Organic Chemicals/analysis/toxicity
MH  - Phospholipids/analysis
MH  - Tetrazolium Salts
MH  - Thiazoles
MH  - Vero Cells
MH  - Water Pollutants, Chemical/chemistry/*toxicity
EDAT- 2010/04/30 06:00
MHDA- 2010/08/13 06:00
CRDT- 2010/04/30 06:00
PHST- 2009/07/17 00:00 [received]
PHST- 2010/03/21 00:00 [revised]
PHST- 2010/03/28 00:00 [accepted]
PHST- 2010/04/30 06:00 [entrez]
PHST- 2010/04/30 06:00 [pubmed]
PHST- 2010/08/13 06:00 [medline]
AID - S0304-3894(10)00419-X [pii]
AID - 10.1016/j.jhazmat.2010.03.113 [doi]
PST - ppublish
SO  - J Hazard Mater. 2010 Jul 15;179(1-3):1055-64. doi: 10.1016/j.jhazmat.2010.03.113. 
      Epub 2010 Apr 2.