PMID- 20427199
OWN - NLM
STAT- MEDLINE
DCOM- 20100901
LR  - 20211020
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 51
IP  - 1
DP  - 2010 Jul
TI  - Differential effects of lactacystin on cytokine production in activated Jurkat 
      cells and murine splenocytes.
PG  - 12-7
LID - 10.1016/j.cyto.2010.03.018 [doi]
AB  - Previous studies have demonstrated that the proteasome inhibitor, lactacystin, 
      suppresses cytokine production and induction of other inflammatory mediators by 
      LPS-stimulated macrophages. The purpose of the present studies was to determine 
      the effect of lactacystin upon the function of activated human Jurkat T cells and 
      murine splenocytes. Lactacystin treatment suppressed interleukin (IL)-2, 
      interferon (IFN)gamma, and IL-13 production similarly in both activated Jurkat 
      cells and primary splenocytes. Interestingly, lactacystin produced differential 
      effects on IL-4 transcription in the two models. While lactacystin inhibited IL-4 
      mRNA transcription in primary splenocytes, it induced IL-4 mRNA in a 
      concentration-dependent manner in Jurkat cells. The increase in IL-4 mRNA levels 
      by lactacystin did not correlate with increases in T(H2)-specific transcription 
      factors, avian musculoaponeurotic fibrosarcoma AS42 oncogene homolog (c-maf) or 
      GATA binding protein 3 (GATA-3). In addition, the binding of both GATA-3 and 
      t-bet to their respective response elements was essentially unchanged by 
      lactacystin treatment in both splenocytes and Jurkat T cells, suggesting the 
      induction of IL-4 is due to other mechanisms. Collectively, the current studies 
      suggest proteasomal activity has differential effects on IL-4 transcription in 
      activated Jurkat cells and primary splenocytes.
CI  - Copyright 2010 Elsevier Ltd. All rights reserved.
FAU - Rockwell, Cheryl E
AU  - Rockwell CE
AD  - Department of Basic Medical Science, School of Medicine, and Shock/Trauma 
      Research Center, University of Missouri Kansas City, Kansas City, MO 64108, USA. 
      crockwell@kumc.edu
FAU - Qureshi, Nilofer
AU  - Qureshi N
LA  - eng
GR  - RR016475/RR/NCRR NIH HHS/United States
GR  - K99 ES018885/ES/NIEHS NIH HHS/United States
GR  - GM050870/GM/NIGMS NIH HHS/United States
GR  - R01 GM050870/GM/NIGMS NIH HHS/United States
GR  - R01 GM050870-17/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100427
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Cytokines)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (Proteasome Inhibitors)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
RN  - 133343-34-7 (lactacystin)
RN  - 9007-49-2 (DNA)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/*analogs & derivatives/pharmacology
MH  - Animals
MH  - Cell Separation
MH  - Cytokines/*biosynthesis
MH  - DNA/metabolism
MH  - GATA3 Transcription Factor/metabolism
MH  - Humans
MH  - Jurkat Cells
MH  - Lymphocyte Activation/*drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Proteasome Inhibitors
MH  - Protein Binding/drug effects
MH  - Spleen/*cytology/*immunology
MH  - T-Box Domain Proteins/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Th2 Cells/drug effects/immunology
PMC - PMC2911229
MID - NIHMS201594
EDAT- 2010/04/30 06:00
MHDA- 2010/09/02 06:00
PMCR- 2011/07/01
CRDT- 2010/04/30 06:00
PHST- 2009/04/09 00:00 [received]
PHST- 2010/01/08 00:00 [revised]
PHST- 2010/03/30 00:00 [accepted]
PHST- 2010/04/30 06:00 [entrez]
PHST- 2010/04/30 06:00 [pubmed]
PHST- 2010/09/02 06:00 [medline]
PHST- 2011/07/01 00:00 [pmc-release]
AID - S1043-4666(10)00092-X [pii]
AID - 10.1016/j.cyto.2010.03.018 [doi]
PST - ppublish
SO  - Cytokine. 2010 Jul;51(1):12-7. doi: 10.1016/j.cyto.2010.03.018. Epub 2010 Apr 27.