PMID- 20428804
OWN - NLM
STAT- MEDLINE
DCOM- 20100930
LR  - 20190606
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 23
IP  - 6
DP  - 2010 Jun
TI  - Anticancer activity of phenoxazines produced by bovine erythrocytes on colon 
      cancer cells.
PG  - 1517-22
AB  - The present study investigated the anticancer activity of 
      2-aminophenoxazine-3-one (Phx-3) and 2-amino-4,4 alpha-dihydro-4 
      alpha,7-dimethyl-3H-phenoxazine-3-one (Phx-1), which were obtained by improved 
      preparation methods using bovine erythrocyte suspension, on colon cancer cell 
      lines COLO201, DLD1 and PMCO1 in vitro. The preparation methods for Phx-1 and 
      Phx-3 had the advantages of extensively shortening reaction time and reducing 
      sample volumes up to one-seventh during treatment, compared with the conventional 
      method using bovine hemoglobin solution, resulting in extensive reduction of 
      handling time. Phx-1 and Phx-3 thus obtained were identified as pure by the 
      absorption spectra and NMR spectra. These phenoxazines exerted strong, 
      dose-dependent anticancer activity against colon cancer cell lines COLO201, DLD1 
      and PMCO1 in vitro and induced apoptosis of these cells. The present results 
      demonstrate that Phx-1 and Phx-3, which were prepared by extensively improved 
      methods using bovine erythrocytes, may be useful as therapeutic drugs against 
      colon cancer that is intractable to chemotherapy.
FAU - Nakachi, Takeshi
AU  - Nakachi T
AD  - Fourth Department of Surgery, Tokyo Medical University, Ibaraki Medical Center, 
      Ibaraki 300-0395, Japan.
FAU - Tabuchi, Takafumi
AU  - Tabuchi T
FAU - Takasaki, Akira
AU  - Takasaki A
FAU - Arai, Sadao
AU  - Arai S
FAU - Miyazawa, Keisuke
AU  - Miyazawa K
FAU - Tomoda, Akio
AU  - Tomoda A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (2-amino-4,4alpha-dihydro-4alpha,7-dimethyl-3H-phenoxazine-3-one)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Oxazines)
RN  - 1916-59-2 (3-aminophenoxazone)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*pharmacology
MH  - Cattle
MH  - Cell Proliferation/*drug effects
MH  - Chromatography, Thin Layer
MH  - Colonic Neoplasms/*drug therapy
MH  - Erythrocytes/*metabolism
MH  - Humans
MH  - Magnetic Resonance Spectroscopy
MH  - Oxazines/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 2010/04/30 06:00
MHDA- 2010/10/01 06:00
CRDT- 2010/04/30 06:00
PHST- 2010/04/30 06:00 [entrez]
PHST- 2010/04/30 06:00 [pubmed]
PHST- 2010/10/01 06:00 [medline]
AID - 10.3892/or_00000790 [doi]
PST - ppublish
SO  - Oncol Rep. 2010 Jun;23(6):1517-22. doi: 10.3892/or_00000790.