PMID- 20428886
OWN - NLM
STAT- MEDLINE
DCOM- 20100622
LR  - 20211020
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 456
IP  - 6
DP  - 2010 Jun
TI  - Extensive biliary intraepithelial neoplasia (BilIN) and multifocal early 
      intrahepatic cholangiocarcinoma in non-biliary cirrhosis.
PG  - 711-7
LID - 10.1007/s00428-010-0899-3 [doi]
AB  - Biliary intraepithelial neoplasia (BilIN), a preneoplastic condition that may 
      precede invasive intrahepatic cholangiocarcinoma (ICC), has been compared to 
      pancreatic intraepithelial neoplasia (PanIN), a precursor lesion of pancreatic 
      carcinoma. Biliary tract carcinoma development and progression is associated with 
      several gene alterations, but BilIN lesions have yet to be studied in detail by 
      molecular techniques. We describe a case of extensive intrahepatic biliary 
      dysplasia, with lesions ranging from BilIN-1 to BilIN-3 lesions, and multifocal 
      microscopic ICC in hepatitis C virus (HCV)- and alcohol-related cirrhosis. The 
      small ICC foci had remained undetected prior to transplantation. Fluorescence in 
      situ hybridization (FISH) analysis was performed on three foci of BilIN-3 lesions 
      and on three microinvasive ICC foci with a combination of three FISH probes 
      directed against genes frequently altered in pancreatic and biliary tract 
      carcinomas. FISH analysis revealed a CDKNA2 heterozygous deletion in one BilIN-3 
      focus, and in one non-contiguous ICC focus, although the deletion was just above 
      the chosen threshold. No deletions were detected in the genomic regions encoding 
      TP53 and SMAD4. This report documents for the first time the development of 
      multifocal ICC in the setting of extensive biliary dysplasia in a patient with 
      three risk factors, HCV infection, alcohol abuse, and cirrhosis, and suggests 
      heterogeneous carcinogenesis in ICC and possible involvement of the CDKNA2 gene.
FAU - Rougemont, Anne-Laure
AU  - Rougemont AL
AD  - Division of Clinical Pathology, Geneva University Hospitals, Rue 
      Gabrielle-Perret-Gentil 4, 1211, Geneva 14, Switzerland. 
      anne-laure.rougemont@hcuge.ch
FAU - Genevay, Muriel
AU  - Genevay M
FAU - McKee, Thomas A
AU  - McKee TA
FAU - Gremaud, Magali
AU  - Gremaud M
FAU - Mentha, Gilles
AU  - Mentha G
FAU - Rubbia-Brandt, Laura
AU  - Rubbia-Brandt L
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20100429
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
SB  - IM
MH  - Adult
MH  - Bile Duct Neoplasms/genetics/*pathology
MH  - *Bile Ducts, Intrahepatic
MH  - Cholangiocarcinoma/genetics/*pathology
MH  - Genes, p16
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Liver Cirrhosis/*pathology
MH  - Male
MH  - Precancerous Conditions/genetics/*pathology
EDAT- 2010/04/30 06:00
MHDA- 2010/06/23 06:00
CRDT- 2010/04/30 06:00
PHST- 2009/09/23 00:00 [received]
PHST- 2010/02/20 00:00 [accepted]
PHST- 2010/01/14 00:00 [revised]
PHST- 2010/04/30 06:00 [entrez]
PHST- 2010/04/30 06:00 [pubmed]
PHST- 2010/06/23 06:00 [medline]
AID - 10.1007/s00428-010-0899-3 [doi]
PST - ppublish
SO  - Virchows Arch. 2010 Jun;456(6):711-7. doi: 10.1007/s00428-010-0899-3. Epub 2010 
      Apr 29.