PMID- 20430358
OWN - NLM
STAT- MEDLINE
DCOM- 20100622
LR  - 20201219
IS  - 1097-6868 (Electronic)
IS  - 0002-9378 (Linking)
VI  - 202
IP  - 6
DP  - 2010 Jun
TI  - Overexpression of miR-152 leads to reduced expression of human leukocyte 
      antigen-G and increased natural killer cell mediated cytolysis in JEG-3 cells.
PG  - 592.e1-7
LID - 10.1016/j.ajog.2010.03.002 [doi]
AB  - OBJECTIVE: The purpose of this study was to gain a further understanding of the 
      relationship between miR-152 and human leukocyte antigen (HLA)-G in human 
      trophoblast cell line (JEG-3). STUDY DESIGN: The JEG-3 cells were transfected 
      with pre-miR-152. The effect of the overexpressed miR-152 on HLA-G expression, 
      trophoblast invasion, and natural killer (NK) cell-mediated cytolysis were 
      assessed by reverse-transcription polymerase chain reaction (RT-PCR) and Western 
      blot analysis, transwell invasion assay, and NK cell cytotoxicity assay, 
      respectively. RESULTS: The miR-152 repressed HLA-G expression but exerted no 
      effect on JEG-3 cell invasion, and overexpression of miR-152 led to increased NK 
      cell-mediated cytolysis in JEG-3 cells. CONCLUSION: The data indicate that 
      miR-152 may function as an immune system enhancer through up-regulating NK 
      cell-mediated cytolysis of host cells.
CI  - Copyright 2010 Mosby, Inc. All rights reserved.
FAU - Zhu, Xiao-ming
AU  - Zhu XM
AD  - Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military 
      Medical University, Xi'an, China. xiaomingzhu1981@hotmail.com
FAU - Han, Tao
AU  - Han T
FAU - Wang, Xiao-hong
AU  - Wang XH
FAU - Li, Yan-hong
AU  - Li YH
FAU - Yang, Hua-guang
AU  - Yang HG
FAU - Luo, Ya-ning
AU  - Luo YN
FAU - Yin, Guo-wu
AU  - Yin GW
FAU - Yao, Yuan-qing
AU  - Yao YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100428
PL  - United States
TA  - Am J Obstet Gynecol
JT  - American journal of obstetrics and gynecology
JID - 0370476
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (MicroRNAs)
RN  - 0 (Untranslated Regions)
SB  - IM
MH  - Analysis of Variance
MH  - Blotting, Western
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cytotoxicity, Immunologic/genetics/immunology
MH  - HLA Antigens/genetics/immunology/*metabolism
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/genetics/immunology/*metabolism
MH  - Humans
MH  - Killer Cells, Natural/immunology/*metabolism
MH  - MicroRNAs/genetics/immunology/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trophoblasts/cytology/immunology/*metabolism
MH  - Untranslated Regions/genetics/immunology
EDAT- 2010/05/01 06:00
MHDA- 2010/06/23 06:00
CRDT- 2010/05/01 06:00
PHST- 2009/09/20 00:00 [received]
PHST- 2010/01/28 00:00 [revised]
PHST- 2010/03/01 00:00 [accepted]
PHST- 2010/05/01 06:00 [entrez]
PHST- 2010/05/01 06:00 [pubmed]
PHST- 2010/06/23 06:00 [medline]
AID - S0002-9378(10)00289-9 [pii]
AID - 10.1016/j.ajog.2010.03.002 [doi]
PST - ppublish
SO  - Am J Obstet Gynecol. 2010 Jun;202(6):592.e1-7. doi: 10.1016/j.ajog.2010.03.002. 
      Epub 2010 Apr 28.