PMID- 20430402
OWN - NLM
STAT- MEDLINE
DCOM- 20101020
LR  - 20211020
IS  - 1873-7994 (Electronic)
IS  - 0021-9924 (Print)
IS  - 0021-9924 (Linking)
VI  - 43
IP  - 4
DP  - 2010 Jul-Aug
TI  - Quantitative PCR analysis of laryngeal muscle fiber types.
PG  - 327-34
LID - 10.1016/j.jcomdis.2010.04.006 [doi]
AB  - Voice and swallowing dysfunction as a result of recurrent laryngeal nerve 
      paralysis can be improved with vocal fold injections or laryngeal framework 
      surgery. However, denervation atrophy can cause late-term clinical failure. A 
      major determinant of skeletal muscle physiology is myosin heavy chain (MyHC) 
      expression, and previous protein analyses have shown changes in laryngeal muscle 
      fiber MyHC isoform with denervation. RNA analyses in this setting have not been 
      performed, and understanding RNA levels will allow interventions better designed 
      to reverse processes such as denervation in the future. Total RNA was extracted 
      from bilateral rat thyroarytenoid (TA), posterior cricoarytenoid (PCA), and 
      cricothyroid (CT) muscles in rats. Primers were designed using published MyHC 
      isoform sequences. SYBR Green real-time reverse transcription-polymerase chain 
      reaction (SYBR-RT-PCR) was used for quantification. The electropherogram showed a 
      clear separation of total RNA to 28S and 18S subunits. Melting curves illustrated 
      single peaks for all type MyHC primers. All MyHC isoforms were identified in all 
      muscles with various degrees of expression. Quantitative PCR is a sensitive 
      method to detect MyHC isoforms in laryngeal muscle. Isoform expression using mRNA 
      analysis was similar to previous analyses but showed some important differences. 
      This technique can be used to quantitatively assess response to interventions 
      targeted to maintain muscle bulk after denervation. LEARNING OUTCOMES: (1) 
      Readers will be able to describe the relationship between myosin heavy chain 
      expression and muscle contractile properties. (2) Readers will be able to 
      separate myosin heavy chain isoforms into slow and fast twitch phenotypes. (3) 
      Readers will be able to describe differential muscle isoform expression between 
      different laryngeal muscles. (4) Readers will be able to compare this study to 
      other modalities of determining muscle fiber type.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Van Daele, Douglas J
AU  - Van Daele DJ
AD  - Department of Otolaryngology - Head and Neck Surgery, Roy J. and Lucille A. 
      Carver College of Medicine, University of Iowa, Iowa City, IA, USA. 
      douglas-van-daele@uiowa.edu
LA  - eng
GR  - R13 DC003383/DC/NIDCD NIH HHS/United States
PT  - Journal Article
DEP - 20100408
PL  - United States
TA  - J Commun Disord
JT  - Journal of communication disorders
JID - 0260316
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - Animals
MH  - Fluorescence
MH  - Laryngeal Muscles/*metabolism
MH  - Muscle, Skeletal/metabolism
MH  - Myosin Heavy Chains/genetics/*metabolism
MH  - Polymerase Chain Reaction/*methods
MH  - Protein Isoforms/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Temperature
MH  - Time Factors
PMC - PMC4530018
MID - NIHMS455793
EDAT- 2010/05/01 06:00
MHDA- 2010/10/21 06:00
PMCR- 2015/08/09
CRDT- 2010/05/01 06:00
PHST- 2010/03/14 00:00 [received]
PHST- 2010/03/21 00:00 [revised]
PHST- 2010/03/23 00:00 [accepted]
PHST- 2010/05/01 06:00 [entrez]
PHST- 2010/05/01 06:00 [pubmed]
PHST- 2010/10/21 06:00 [medline]
PHST- 2015/08/09 00:00 [pmc-release]
AID - S0021-9924(10)00024-9 [pii]
AID - 10.1016/j.jcomdis.2010.04.006 [doi]
PST - ppublish
SO  - J Commun Disord. 2010 Jul-Aug;43(4):327-34. doi: 10.1016/j.jcomdis.2010.04.006. 
      Epub 2010 Apr 8.