PMID- 20430635
OWN - NLM
STAT- MEDLINE
DCOM- 20100915
LR  - 20220330
IS  - 1878-4291 (Electronic)
IS  - 0968-4328 (Linking)
VI  - 41
IP  - 6
DP  - 2010 Aug
TI  - Interaction of Candida parapsilosis isolates with human hair and nail surfaces 
      revealed by scanning electron microscopy analysis.
PG  - 604-8
LID - 10.1016/j.micron.2010.03.011 [doi]
AB  - Candida parapsilosis is found frequently as commensal organism on epithelial 
      tissues, and is also an increasing cause of nosocomial infection. Scanning 
      electron microscope (SEM) observations were used to analyse the capability of C. 
      parapsilosis cells to adhere and grow as biofilm on human natural substrates and 
      to compare the adherence pattern of isolates exhibiting distinct phenotypes. 
      Cells from the crepe phenotype are predominantly elongated and form pseudohyphae 
      whereas cells from the smooth phenotype are yeast-shaped, either in liquid 
      cultures or on human nail and hair surfaces. The electron micrographs revealed 
      that C. parapsilosis cells from the smooth phenotype adhered in higher number to 
      both surfaces compared to the observed for the crepe phenotype. SEM analysis of 
      human hair surface revealed that cells from the smooth phenotype appear as 
      clumped blastoconidia of uniform morphology embedded in a flocculent 
      extracellular material forming biofilm. The extracellular material and biofilm 
      were seeing in a less extension in the crepe phenotype. A distinct adherence 
      pattern was observed when human nail was used as substrate. Here C. parapsilosis 
      cells seem to be linked to surface structures of human nail plate. Fibrillar 
      extracellular material was observed connecting neighbouring cells as well as nail 
      surface.
CI  - (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Oliveira, Marcelo T
AU  - Oliveira MT
AD  - Centro de Ciencias Biologicas, Universidade Estadual de Londrina, Londrina, PR, 
      Brazil.
FAU - Specian, Ana Flavia L
AU  - Specian AF
FAU - Andrade, Celia G T J
AU  - Andrade CG
FAU - Franca, Emanuele J G
AU  - Franca EJ
FAU - Furlaneto-Maia, Luciana
AU  - Furlaneto-Maia L
FAU - Furlaneto, Marcia C
AU  - Furlaneto MC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100402
PL  - England
TA  - Micron
JT  - Micron (Oxford, England : 1993)
JID - 9312850
SB  - IM
MH  - Biofilms/growth & development
MH  - Candida/*ultrastructure
MH  - Cell Adhesion
MH  - Hair/*microbiology
MH  - *Host-Pathogen Interactions
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - Nails/*microbiology
EDAT- 2010/05/01 06:00
MHDA- 2010/09/16 06:00
CRDT- 2010/05/01 06:00
PHST- 2010/02/11 00:00 [received]
PHST- 2010/03/22 00:00 [revised]
PHST- 2010/03/27 00:00 [accepted]
PHST- 2010/05/01 06:00 [entrez]
PHST- 2010/05/01 06:00 [pubmed]
PHST- 2010/09/16 06:00 [medline]
AID - S0968-4328(10)00064-8 [pii]
AID - 10.1016/j.micron.2010.03.011 [doi]
PST - ppublish
SO  - Micron. 2010 Aug;41(6):604-8. doi: 10.1016/j.micron.2010.03.011. Epub 2010 Apr 2.