PMID- 20434119
OWN - NLM
STAT- MEDLINE
DCOM- 20100915
LR  - 20160928
IS  - 1995-9133 (Electronic)
IS  - 1684-1182 (Linking)
VI  - 43
IP  - 1
DP  - 2010 Feb
TI  - Changing cytokine patterns in systemic lupus: a prospective longitudinal study.
PG  - 18-25
LID - 10.1016/S1684-1182(10)60003-5 [doi]
AB  - BACKGROUND/PURPOSE: We have previously reported that lupus monocytes display 
      distinctively differing patterns of C-reactive protein (CRP)-inducing cytokine 
      interleukin (IL)-6, IL-1beta, and tumor necrosis factor (TNF)-alpha secretion 
      when stimulated with either immune complexes (ICs) or lipopolysaccharide (LPS). 
      In this study, we investigated whether the cytokine patterns of peripheral blood 
      mononuclear cells (PBMCs) isolated from lupus patients acquired an IC or LPS 
      pattern, either over time, or following corticosteroid or hydroxychloroquine use. 
      METHODS: PBMCs from lupus patients were obtained at 0, 1, 3, and 6 months post 
      diagnosis and stimulated with ICs or LPS. Cells were obtained for polymerase 
      chain reaction to determine the IL-6, IL-1beta, and TNF-alpha mRNA expression, 
      and were assigned as having acquired either an IC or an LPS pattern. RESULTS: 
      Upon stimulation, the mRNA expression levels of the IL-6 and IL-1beta were 
      significantly higher in IC-pattern PBMCs than in LPS-pattern PBMCs (p= 0.021 and 
      0.028, respectively). Consistent with this, serum CRP levels in the IC-pattern 
      group were significantly higher than those in the LPS-pattern groups (p = 0.027). 
      Total serum CRP levels were positively correlated with serum C3c and C4 
      concentrations, and inversely correlated with serum anti-double stranded DNA 
      (anti-dsDNA) levels. Conversely, circulating ICs were positively correlated with 
      serum anti-dsDNA levels and inversely correlated with serum C4 concentrations. 
      CONCLUSION: Within the same individual, the CRP-inducing cytokine patterns can be 
      changed, either naturally or after medication. Pre-existing serum circulating ICs 
      are not predisposed to either IC or LPS cytokine patterns. Finally, CRP levels 
      were correlated with anti-dsDNA consumption.
FAU - Liou, Lieh-Bang
AU  - Liou LB
AD  - Division of Rheumatology, Allergy and Immunology, Chang Gung Memorial Hospital at 
      Lin-kou and Chang Gung University College of Medicine, Tao-yuan, Taiwan. 
      b890121@adm.cgmh.org.tw
FAU - Chao, Wan-Ju
AU  - Chao WJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100329
PL  - England
TA  - J Microbiol Immunol Infect
JT  - Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
JID - 100956211
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 4QWG6N8QKH (Hydroxychloroquine)
SB  - IM
EIN - J Microbiol Immunol Infect. 2011 Feb;44(1):77. PMID: 25306136
MH  - Adrenal Cortex Hormones/therapeutic use
MH  - Anti-Inflammatory Agents/therapeutic use
MH  - Antigen-Antibody Complex/immunology
MH  - Cells, Cultured
MH  - Cytokines/*biosynthesis
MH  - Gene Expression Profiling
MH  - Humans
MH  - Hydroxychloroquine/therapeutic use
MH  - Leukocytes, Mononuclear/*immunology
MH  - Lipopolysaccharides/immunology
MH  - Longitudinal Studies
MH  - Lupus Erythematosus, Systemic/drug therapy/*immunology
MH  - Prospective Studies
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2010/05/04 06:00
MHDA- 2010/09/16 06:00
CRDT- 2010/05/04 06:00
PHST- 2008/07/11 00:00 [received]
PHST- 2008/08/19 00:00 [revised]
PHST- 2009/02/13 00:00 [accepted]
PHST- 2010/05/04 06:00 [entrez]
PHST- 2010/05/04 06:00 [pubmed]
PHST- 2010/09/16 06:00 [medline]
AID - S1684-1182(10)60003-5 [pii]
AID - 10.1016/S1684-1182(10)60003-5 [doi]
PST - ppublish
SO  - J Microbiol Immunol Infect. 2010 Feb;43(1):18-25. doi: 
      10.1016/S1684-1182(10)60003-5. Epub 2010 Mar 29.