PMID- 20434160
OWN - NLM
STAT- MEDLINE
DCOM- 20100826
LR  - 20181201
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1217
IP  - 23
DP  - 2010 Jun 4
TI  - Differently complex oligosaccharides can be easily identified by matrix-assisted 
      laser desorption and ionization time-of-flight mass spectrometry directly from a 
      standard thin-layer chromatography plate.
PG  - 3711-5
LID - 10.1016/j.chroma.2010.04.005 [doi]
AB  - Thin-layer chromatography (TLC) is a simple, fast and inexpensive separation 
      method. Unambiguous identification of the TLC spots is, however, often a problem. 
      Here we show for the first time that oligosaccharides (derived from dextran, 
      alginate, hyaluronan and chondroitin sulfate) can be characterized by 
      matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass 
      spectrometry (MS) directly on a TLC plate. The applied oligosaccharides were 
      either commercially available or obtained from the polysaccharides by HCl-induced 
      hydrolysis. Normal phase TLC was followed by MALDI-TOF MS subsequent to matrix 
      deposition. It will be shown that high quality mass spectra can be obtained that 
      enable unequivocal assignments. It will also be shown that the high content of 
      formic acid in the solvent system does not confer major problems but is 
      responsible for the partial formylation of the analyte and minor N-acetyl loss 
      from hyaluronan and chondroitin sulfate.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Nimptsch, K
AU  - Nimptsch K
AD  - University of Leipzig, Medical Faculty, Institute of Medical Physics and 
      Biophysics, Hartelstr. 16-18, D-04107 Leipzig, Germany.
FAU - Suss, R
AU  - Suss R
FAU - Riemer, T
AU  - Riemer T
FAU - Nimptsch, A
AU  - Nimptsch A
FAU - Schnabelrauch, M
AU  - Schnabelrauch M
FAU - Schiller, J
AU  - Schiller J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100413
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Alginates)
RN  - 0 (Dextrans)
RN  - 0 (Hexuronic Acids)
RN  - 0 (Oligosaccharides)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - 9007-27-6 (Chondroitin)
RN  - QTT17582CB (Hydrochloric Acid)
SB  - IM
MH  - Alginates/chemistry
MH  - Chondroitin/chemistry
MH  - Chromatography, Thin Layer/*methods
MH  - Dextrans/chemistry
MH  - Glucuronic Acid/chemistry
MH  - Hexuronic Acids/chemistry
MH  - Hyaluronic Acid/chemistry
MH  - Hydrochloric Acid/chemistry
MH  - Hydrolysis
MH  - Oligosaccharides/*chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
EDAT- 2010/05/04 06:00
MHDA- 2010/08/27 06:00
CRDT- 2010/05/04 06:00
PHST- 2010/01/22 00:00 [received]
PHST- 2010/03/29 00:00 [revised]
PHST- 2010/04/06 00:00 [accepted]
PHST- 2010/05/04 06:00 [entrez]
PHST- 2010/05/04 06:00 [pubmed]
PHST- 2010/08/27 06:00 [medline]
AID - S0021-9673(10)00473-5 [pii]
AID - 10.1016/j.chroma.2010.04.005 [doi]
PST - ppublish
SO  - J Chromatogr A. 2010 Jun 4;1217(23):3711-5. doi: 10.1016/j.chroma.2010.04.005. 
      Epub 2010 Apr 13.