PMID- 20435028
OWN - NLM
STAT- MEDLINE
DCOM- 20100830
LR  - 20211020
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 344
IP  - 2
DP  - 2010 Aug 15
TI  - Non-additive interactions involving two distinct elements mediate sloppy-paired 
      regulation by pair-rule transcription factors.
PG  - 1048-59
LID - 10.1016/j.ydbio.2010.04.026 [doi]
AB  - The relatively simple combinatorial rules responsible for establishing the 
      initial metameric expression of sloppy-paired-1 (slp1) in the Drosophila 
      blastoderm embryo make this system an attractive model for investigating the 
      mechanism of regulation by pair-rule transcription factors. This investigation of 
      slp1 cis-regulatory architecture identifies two distinct elements, a proximal 
      early stripe element (PESE) and a distal early stripe element (DESE) located from 
      -3.1kb to -2.5kb and from -8.1kb to -7.1kb upstream of the slp1 promoter, 
      respectively, that mediate this early regulation. The proximal element expresses 
      only even-numbered stripes and mediates repression by Even-skipped (Eve) as well 
      as by the combination of Runt and Fushi-tarazu (Ftz). A 272 basepair sub-element 
      of PESE retains an Eve-dependent repression, but is expressed throughout the 
      even-numbered parasegments due to the loss of repression by Runt and Ftz. In 
      contrast, the distal element expresses both odd and even-numbered stripes and 
      also drives inappropriate expression in the anterior half of the odd-numbered 
      parasegments due to an inability to respond to repression by Eve. Importantly, a 
      composite reporter gene containing both early stripe elements recapitulates 
      pair-rule gene-dependent regulation in a manner beyond what is expected from 
      combining their individual patterns. These results indicate that interactions 
      involving distinct cis-elements contribute to the proper integration of pair-rule 
      regulatory information. A model fully accounting for these results proposes that 
      metameric slp1 expression is achieved through the Runt-dependent regulation of 
      interactions between these two pair-rule response elements and the slp1 promoter.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Prazak, Lisa
AU  - Prazak L
AD  - Department of Biochemistry and Cell Biology and the Center for Developmental 
      Genetics, Stony Brook University, Stony Brook, NY 11794-5215, USA.
FAU - Fujioka, Miki
AU  - Fujioka M
FAU - Gergen, J Peter
AU  - Gergen JP
LA  - eng
GR  - R01 GM050231/GM/NIGMS NIH HHS/United States
GR  - R01 GM050231-12/GM/NIGMS NIH HHS/United States
GR  - R01GM050231/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100506
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Blastoderm/metabolism/physiology
MH  - Drosophila/*genetics/metabolism
MH  - Embryo, Nonmammalian
MH  - *Gene Expression Regulation
MH  - Regulatory Sequences, Nucleic Acid
MH  - *Transcription Factors/genetics/metabolism/physiology
PMC - PMC2914134
MID - NIHMS207834
EDAT- 2010/05/04 06:00
MHDA- 2010/08/31 06:00
PMCR- 2011/08/15
CRDT- 2010/05/04 06:00
PHST- 2009/10/22 00:00 [received]
PHST- 2010/04/08 00:00 [revised]
PHST- 2010/04/23 00:00 [accepted]
PHST- 2010/05/04 06:00 [entrez]
PHST- 2010/05/04 06:00 [pubmed]
PHST- 2010/08/31 06:00 [medline]
PHST- 2011/08/15 00:00 [pmc-release]
AID - S0012-1606(10)00271-X [pii]
AID - 10.1016/j.ydbio.2010.04.026 [doi]
PST - ppublish
SO  - Dev Biol. 2010 Aug 15;344(2):1048-59. doi: 10.1016/j.ydbio.2010.04.026. Epub 2010 
      May 6.