PMID- 20435460
OWN - NLM
STAT- MEDLINE
DCOM- 20100930
LR  - 20131121
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 25
IP  - 11
DP  - 2010 Jul 15
TI  - Direct growth of nanoporous Au and its application in electrochemical biosensing.
PG  - 2458-63
LID - 10.1016/j.bios.2010.04.006 [doi]
AB  - In this work, we have directly grown three-dimensional nanoporous Au networks 
      onto a Ti substrate using the hydrothermal technique. This newly designed 
      material with a large surface area was used as a supporting matrix for 
      immobilizing a redox protein, hemoglobin (Hb), to develop a high-performance 
      hydrogen peroxide biosensor. Scanning electron microscope (SEM), X-ray 
      photoelectron spectroscopy (XPS), and energy-dispersive X-ray (EDX) spectroscopy 
      were employed to characterize the morphology and composition of the fabricated 
      nanoporous Au network. Cyclic voltammetry (CV) and amperometry were used to study 
      and to optimize the performance of the fabricated electrochemical biosensor. Our 
      CV studies show the direct electron transfer of Hb immobilized on the nanoporous 
      Au network. In addition, amperometric H(2)O(2) sensing experiments revealed that 
      the nanoporous Au-network based biosensor exhibits fast response, long linearity, 
      a low detection limit, high stability and very good reproducibility. Under the 
      optimized conditions, the linearity of the developed biosensor for the detection 
      of H(2)O(2) spans from 5 x 10(-8) to 2 x 10(-4)M with a detection limit of 2 x 
      10(-8)M (based on S/N=3).
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Kafi, A K M
AU  - Kafi AK
AD  - Department of Chemistry, Lakehead University, Thunder Bay, Ontario P7B 5E1, 
      Canada.
FAU - Ahmadalinezhad, Asieh
AU  - Ahmadalinezhad A
FAU - Wang, Jingpeng
AU  - Wang J
FAU - Thomas, Dan F
AU  - Thomas DF
FAU - Chen, Aicheng
AU  - Chen A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100413
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Hemoglobins)
RN  - 7440-57-5 (Gold)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Adsorption
MH  - Biosensing Techniques/*instrumentation
MH  - Conductometry/*instrumentation
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Gold/*chemistry
MH  - Hemoglobins/*chemistry
MH  - Hydrogen Peroxide/*analysis/chemistry
MH  - Nanostructures/*chemistry/ultrastructure
MH  - Nanotechnology/*instrumentation
MH  - Porosity
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2010/05/04 06:00
MHDA- 2010/10/01 06:00
CRDT- 2010/05/04 06:00
PHST- 2010/03/21 00:00 [received]
PHST- 2010/04/02 00:00 [revised]
PHST- 2010/04/06 00:00 [accepted]
PHST- 2010/05/04 06:00 [entrez]
PHST- 2010/05/04 06:00 [pubmed]
PHST- 2010/10/01 06:00 [medline]
AID - S0956-5663(10)00176-4 [pii]
AID - 10.1016/j.bios.2010.04.006 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2010 Jul 15;25(11):2458-63. doi: 10.1016/j.bios.2010.04.006. 
      Epub 2010 Apr 13.