PMID- 20436789
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1742-6413 (Electronic)
IS  - 0974-5963 (Print)
IS  - 1742-6413 (Linking)
VI  - 7
DP  - 2010 Apr 6
TI  - Fine needle aspiration biopsy diagnosis of dedifferentiated liposarcoma: 
      Cytomorphology and MDM2 amplification by FISH.
PG  - 5
LID - 10.4103/1742-6413.62257 [doi]
LID - 5
AB  - Lipomatous mesenchymal tumors constitute the most common type of soft tissue 
      tumors. Well-differentiated liposarcoma (WDLS) can undergo dedifferentiation to a 
      nonlipogenic sarcoma of variable histologic grade. In the recent literature, 
      amplification of the murine double minute 2 (MDM2) oncogene, which has a role in 
      cell cycle control, has been successful in distinguishing WDLS from benign 
      lesions. We present a case of dedifferentiated liposarcoma diagnosed by 
      fine-needle aspiration (FNA), using cytomorphology and ancillary studies 
      (immunocytochemistry and fluorescent in-situ hybridization). An 85-year old 
      female presented to our institution with a firm soft tissue mass of the right 
      buttock. The FNA showed atypical spindle cells, osteoclast-like giant cells and 
      extracellular dense matrix material. The cell block showed cellular groups of 
      highly atypical spindle cells with osteoid and adipose tissue. Fluorescence in 
      situ hybridization (FISH) studies performed on the cell block demonstrated 
      amplification of the MDM2 gene. In addition, the findings were morphologically 
      compatible with the previously resected retroperitoneal dedifferentiated 
      liposarcoma with areas of osteosarcoma. This rare case illustrates the usefulness 
      of FNA and ancillary studies in the diagnosis and subclassification of soft 
      tissue tumors. To the best of our knowledge, this is the first report of MDM2 
      FISH positivity in a liposarcoma diagnosed by FNA.
FAU - Al-Maghraby, Hatem Q
AU  - Al-Maghraby HQ
AD  - Department of Pathobiology and Laboratory Medicine, University of Toronto, 
      Toronto, Ontario, Canada.
FAU - Khalbuss, Walid E
AU  - Khalbuss WE
FAU - Rao, Uma N M
AU  - Rao UN
FAU - Cieply, Kathleen
AU  - Cieply K
FAU - Dacic, Sanja
AU  - Dacic S
FAU - Monaco, Sara E
AU  - Monaco SE
LA  - eng
PT  - Case Reports
DEP - 20100406
PL  - United States
TA  - Cytojournal
JT  - CytoJournal
JID - 101231642
PMC - PMC2861822
OTO - NOTNLM
OT  - Cytopathology
OT  - MDM2
OT  - fine needle aspiration biopsy liposarcoma
EDAT- 2010/05/04 06:00
MHDA- 2010/05/04 06:01
PMCR- 2010/04/06
CRDT- 2010/05/04 06:00
PHST- 2009/11/10 00:00 [received]
PHST- 2009/12/25 00:00 [accepted]
PHST- 2010/05/04 06:00 [entrez]
PHST- 2010/05/04 06:00 [pubmed]
PHST- 2010/05/04 06:01 [medline]
PHST- 2010/04/06 00:00 [pmc-release]
AID - CJ-07-5 [pii]
AID - 10.4103/1742-6413.62257 [doi]
PST - epublish
SO  - Cytojournal. 2010 Apr 6;7:5. doi: 10.4103/1742-6413.62257.