PMID- 20441969
OWN - NLM
STAT- MEDLINE
DCOM- 20101021
LR  - 20100505
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 81
IP  - 4-5
DP  - 2010 Jun 15
TI  - Ultra-sensitive detection of IgE using biofunctionalized nanoparticle-enhanced 
      SPR.
PG  - 1755-9
LID - 10.1016/j.talanta.2010.03.036 [doi]
AB  - This paper describes an ultra-sensitive surface-based detection method using 
      nanoparticle-enhanced surface plasmon resonance (SPR) for the detection of 
      immunoglobulin E (IgE) proteins, which could potentially be used for the 
      diagnosis of allergic diseases. Two different probes, anti-IgE and IgE specific 
      aptamers, which can specifically interact with IgE at different epitopes were 
      first investigated for their specific interaction with IgE using SPR. Langmuir 
      adsorption coefficient (K(ads)) values were measured as 2.0(+/-0.22)x10(8)M(-1) 
      and 2.2(+/-0.20)x10(8)M(-1) for IgE interactions with anti-IgE and IgE specific 
      aptamers, respectively. The SPR detection limit of the simple adsorption of IgE 
      onto either anti-IgE or IgE specific aptamers was found to be about 1nM. In order 
      to improve the SPR detection signal for IgE, two different approaches utilizing 
      surface formed sandwich complexes with biofunctionalized gold nanoparticles 
      (Au-Nps) were designed and their detection performance were compared; the 
      complexes were created via the adsorption of IgE onto (i) surface immobilized 
      anti-IgE followed by the adsorption of IgE specific aptamer coated gold 
      nanoparticles and (ii) IgE specific aptamer surface with the subsequent 
      adsorption of anti-IgE coated gold nanoparticles. Both detection schemes were 
      able to directly measure IgE at femtomolar concentrations.
FAU - Kim, Suhee
AU  - Kim S
AD  - Department of Chemistry, Kyungpook National University, Daegu, Republic of Korea.
FAU - Lee, Jaeyoung
AU  - Lee J
FAU - Lee, Seok Jae
AU  - Lee SJ
FAU - Lee, Hye Jin
AU  - Lee HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100325
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Epitopes)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Adsorption
MH  - Binding Sites
MH  - Epitopes/chemistry
MH  - Gold/chemistry
MH  - Humans
MH  - Hypersensitivity/immunology
MH  - Immunoglobulin E/*chemistry/*immunology
MH  - Kinetics
MH  - Limit of Detection
MH  - Metal Nanoparticles/chemistry
MH  - Nanoparticles/*chemistry
MH  - Surface Plasmon Resonance/*methods
MH  - Surface Properties
MH  - Time Factors
EDAT- 2010/05/06 06:00
MHDA- 2010/10/22 06:00
CRDT- 2010/05/06 06:00
PHST- 2010/01/21 00:00 [received]
PHST- 2010/03/18 00:00 [revised]
PHST- 2010/03/18 00:00 [accepted]
PHST- 2010/05/06 06:00 [entrez]
PHST- 2010/05/06 06:00 [pubmed]
PHST- 2010/10/22 06:00 [medline]
AID - S0039-9140(10)00230-4 [pii]
AID - 10.1016/j.talanta.2010.03.036 [doi]
PST - ppublish
SO  - Talanta. 2010 Jun 15;81(4-5):1755-9. doi: 10.1016/j.talanta.2010.03.036. Epub 
      2010 Mar 25.