PMID- 2044218
OWN - NLM
STAT- MEDLINE
DCOM- 19910715
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 84
IP  - 3
DP  - 1991 Jun
TI  - Are cord blood B cells functionally mature?
PG  - 389-94
AB  - Very low immunoglobulin secretion occurs in pokeweed mitogen (PWM) stimulated 
      cord blood mononuclear cells (MNC) and has been attributed to an 'immaturity' of 
      both T and B cells of the newborn. The cord blood T cells are phenotypically 
      'naive' cells, in which suppressor activity for B cell function appears to 
      dominate over helper activity. The cord blood B cells, in spite of their 
      expression of different membrane immunoglobulin isotypes, secrete almost no IgG 
      and IgA in the various B cell assays so far compared. We found that cord blood B 
      cells are as competent as B cells from adults to generate clonal IgM, IgG and IgA 
      responses in a culture system in which a cell contact with mutant EL-4 thymoma 
      cells in conjunction with T cell supernatant leads to strong B cell activity. As 
      regarding the possible causes of the low cord blood PWM response, we studied the 
      role of transforming growth factor-beta 1 (TGF-beta 1), a potent inhibitor of 
      lymphocyte functions. TGF-beta 1 sensitivity of B cells and TGF-beta 1 mRNA 
      levels in MNC were found to be similar for adult and cord blood cells. A 
      neutralizing anti-TGF-beta 1 antibody enhance the adult PWM response, but the 
      immunoglobulin secretion in cord MNC remained very low. We conclude that 
      suppression by endogenous TGF-beta 1 occurs in the PWM system but is not 
      responsible for the low immunoglobulin response of cord blood MNC and that the 
      newborn's B cell 'immaturity' can be overcome with potent T cell signals in 
      vitro. This is consistent with the newborn's capacity to generate a T-dependent B 
      cell response in vivo.
FAU - Tucci, A
AU  - Tucci A
AD  - Department of Medicine, Geneva University Hospital, Switzerland.
FAU - Mouzaki, A
AU  - Mouzaki A
FAU - James, H
AU  - James H
FAU - Bonnefoy, J Y
AU  - Bonnefoy JY
FAU - Zubler, R H
AU  - Zubler RH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - B-Lymphocytes/*physiology
MH  - Fetal Blood/*immunology
MH  - Humans
MH  - Immunoglobulin A/biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Immunoglobulin M/biosynthesis
MH  - Infant, Newborn
MH  - Lymphocyte Activation/drug effects
MH  - RNA, Messenger/analysis
MH  - T-Lymphocytes/physiology
MH  - Transforming Growth Factor beta/genetics/physiology
PMC - PMC1535446
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
PMCR- 1992/06/01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
PHST- 1992/06/01 00:00 [pmc-release]
PST - ppublish
SO  - Clin Exp Immunol. 1991 Jun;84(3):389-94.