PMID- 20442265
OWN - NLM
STAT- MEDLINE
DCOM- 20101004
LR  - 20211020
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Print)
IS  - 0022-3751 (Linking)
VI  - 588
IP  - Pt 12
DP  - 2010 Jun 15
TI  - Acid-sensing ion channels in rat hypothalamic vasopressin neurons of the 
      supraoptic nucleus.
PG  - 2147-62
LID - 10.1113/jphysiol.2010.187625 [doi]
AB  - Body fluid balance requires the release of arginine vasopressin (AVP) from the 
      neurohypophysis. The hypothalamic supraoptic nucleus (SON) is a major site of AVP 
      synthesis, and AVP release is controlled somatodendritically or at the level of 
      nerve terminals by electrical activities of magnocellular neurosecretory cells 
      (MNCs). Acid-sensing ion channels (ASICs) are neuronal voltage-insensitive 
      cationic channels that are activated by extracellular acidification. Although 
      ASICs are widely expressed in the central nervous system, functional ASICs have 
      not been assessed in AVP neurons. ASICs are modulated by lactate (La(-)), which 
      reduces the extracellular calcium ion concentration. We hypothesize that ASICs 
      modify neuronal function through La(-) that is generated during local hypoxia 
      resulting from osmotic stimulation in the SON. In the present study, we used the 
      whole-cell patch-clamp technique to show that acid-induced ASIC current is 
      enhanced by La(-) in isolated rat SON MNCs that express an AVP-enhanced green 
      fluorescent protein (eGFP) transgene. Immunohistochemistry and multi-cell reverse 
      transcriptase-polymerase chain reaction experiments revealed that these neurons 
      express the ASIC1a and ASIC2a subunits. In addition, increased La(-) production 
      was specifically observed in the SON after osmotic stress. These results suggest 
      that interaction between ASICs and La(-) in the SON plays an important role in 
      the regulatory mechanism of body fluid homeostasis.
FAU - Ohbuchi, Toyoaki
AU  - Ohbuchi T
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
FAU - Sato, Kaori
AU  - Sato K
FAU - Suzuki, Hideaki
AU  - Suzuki H
FAU - Okada, Yasunobu
AU  - Okada Y
FAU - Dayanithi, Govindan
AU  - Dayanithi G
FAU - Murphy, David
AU  - Murphy D
FAU - Ueta, Yoichi
AU  - Ueta Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100504
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Acid Sensing Ion Channels)
RN  - 0 (Asic2 protein, rat)
RN  - 0 (Degenerin Sodium Channels)
RN  - 0 (Epithelial Sodium Channels)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sodium Channels)
RN  - 0 (Sodium Chloride, Dietary)
RN  - 0 (enhanced green fluorescent protein)
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 33X04XA5AT (Lactic Acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acid Sensing Ion Channels
MH  - Animals
MH  - Arginine Vasopressin/*metabolism
MH  - Calcium/metabolism
MH  - Cell Hypoxia
MH  - Cells, Cultured
MH  - Degenerin Sodium Channels
MH  - Dehydration/metabolism
MH  - Epithelial Sodium Channels/genetics/*metabolism
MH  - Green Fluorescent Proteins/biosynthesis/genetics
MH  - Hydrogen-Ion Concentration
MH  - Immunohistochemistry
MH  - Lactic Acid/metabolism
MH  - Male
MH  - Membrane Potentials
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Neurons/*metabolism
MH  - Osmotic Pressure
MH  - Patch-Clamp Techniques
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Transgenic
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sodium Channels/genetics/*metabolism
MH  - Sodium Chloride, Dietary/administration & dosage
MH  - Supraoptic Nucleus/cytology/*metabolism
MH  - Transcription, Genetic
MH  - *Water-Electrolyte Balance
PMC - PMC2911217
EDAT- 2010/05/06 06:00
MHDA- 2010/10/05 06:00
PMCR- 2011/06/15
CRDT- 2010/05/06 06:00
PHST- 2010/05/06 06:00 [entrez]
PHST- 2010/05/06 06:00 [pubmed]
PHST- 2010/10/05 06:00 [medline]
PHST- 2011/06/15 00:00 [pmc-release]
AID - jphysiol.2010.187625 [pii]
AID - 10.1113/jphysiol.2010.187625 [doi]
PST - ppublish
SO  - J Physiol. 2010 Jun 15;588(Pt 12):2147-62. doi: 10.1113/jphysiol.2010.187625. 
      Epub 2010 May 4.