PMID- 20442679
OWN - NLM
STAT- MEDLINE
DCOM- 20110113
LR  - 20131121
IS  - 1536-4828 (Electronic)
IS  - 0885-3177 (Linking)
VI  - 39
IP  - 7
DP  - 2010 Oct
TI  - Expression of NOD2 in a rat model of acute pancreatitis.
PG  - 1034-40
LID - 10.1097/MPA.0b013e3181da0f1d [doi]
AB  - OBJECTIVES: To observe the expression of nucleotide-binding oligomerization 
      domain 2 (NOD2) in rats with acute pancreatitis (AP). METHODS: Sprague-Dawley 
      rats were randomly divided into sham operation (SO) groups and AP groups. Acute 
      pancreatitis was induced with retrograde infusion of sodium taurocholate into the 
      biliopancreatic duct. They were then killed at 3, 6, 12, 24, and 48 hours after 
      induction of AP. Blood biochemical indicators were detected with automatic 
      biochemistry analyzer. Nuclear factor-kappaB (NF-kappaB) was measured by 
      immunohistochemistry. The NOD2 was detected by real-time quantitative polymerase 
      chain reaction and Western blot. Tumor necrosis factor-alpha (TNF-alpha) was determined 
      by enzyme-linked immunosorbent assay. RESULTS: Compared with the SO group, the 
      level of messenger RNA and protein of NOD2 in pancreatic tissue and peritoneal 
      white blood cells (PWBCs) in the AP groups significantly declined (P < 0.05). The 
      messenger RNA level of NOD2 in the AP groups was correlated positively with 
      amylase (P < 0.05) and negatively with TNF-alpha (P < 0.05); TNF-alpha significantly 
      decreased in the AP groups, whereas NF-kappaB significantly increased (P < 0.05). 
      CONCLUSIONS: The NOD2 may play an important role in the up-regulation and 
      activation of NF-kappaB during inflammation reactions in AP.
FAU - Qian, Mingping
AU  - Qian M
AD  - Department of General Surgery, Shanghai 10th People's Hospital, Tongji University 
      School of Medicine, Shanghai, China.
FAU - Fang, Lin
AU  - Fang L
FAU - Cui, Yingyu
AU  - Cui Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (NOD2 protein, rat)
RN  - 0 (Nod2 Signaling Adaptor Protein)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - EC 3.2.1.- (Amylases)
RN  - RFM9X3LJ49 (Bilirubin)
SB  - IM
MH  - Acute Disease
MH  - Amylases/blood
MH  - Animals
MH  - Bilirubin/blood
MH  - C-Reactive Protein/analysis
MH  - Disease Models, Animal
MH  - Immunohistochemistry
MH  - Interleukin-4/blood
MH  - Intracellular Signaling Peptides and Proteins/*genetics/physiology
MH  - Male
MH  - NF-kappa B/analysis
MH  - Nod2 Signaling Adaptor Protein
MH  - Pancreatitis/*metabolism
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tumor Necrosis Factor-alpha/blood
EDAT- 2010/05/06 06:00
MHDA- 2011/01/14 06:00
CRDT- 2010/05/06 06:00
PHST- 2010/05/06 06:00 [entrez]
PHST- 2010/05/06 06:00 [pubmed]
PHST- 2011/01/14 06:00 [medline]
AID - 10.1097/MPA.0b013e3181da0f1d [doi]
PST - ppublish
SO  - Pancreas. 2010 Oct;39(7):1034-40. doi: 10.1097/MPA.0b013e3181da0f1d.