PMID- 20443213
OWN - NLM
STAT- MEDLINE
DCOM- 20100823
LR  - 20211020
IS  - 1862-1783 (Electronic)
IS  - 1673-1581 (Print)
IS  - 1673-1581 (Linking)
VI  - 11
IP  - 5
DP  - 2010 May
TI  - Overexpression of the steroidogenic acute regulatory protein increases the 
      expression of ATP-binding cassette transporters in microvascular endothelial 
      cells (bEnd.3).
PG  - 350-6
LID - 10.1631/jzus.B0900369 [doi]
AB  - OBJECTIVE: To determine the effect of steroidogenic acute regulatory protein 
      (StAR) overexpression on the levels of adenosine triphosphate (ATP)-binding 
      cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) 
      in an endothelial cell line (bEnd.3). METHODS: The StAR gene was induced in 
      bEnd.3 cells with adenovirus infection. The infection efficiency was detected by 
      fluorescence activated cell sorter (FACS) and fluorescence microscopy. The 
      expressions of StAR gene and protein levels were detected by real-time polymerase 
      chain reaction (PCR) and Western blot. The gene and protein levels of ABCA1 and 
      ABCG1 were detected by real-time PCR and Western blot after StAR overexpression. 
      RESULTS: The result shows that StAR was successfully overexpressed in bEnd.3 
      cells by adenovirus infection. The mRNA and protein expressions of ABCA1 and 
      ABCG1 were greatly increased by StAR overexpression in bEnd.3 cells. CONCLUSION: 
      Overexpression of StAR increases ABCA1 and ABCG1 expressions in endothelial 
      cells.
FAU - Ning, Yan-Xia
AU  - Ning YX
AD  - Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan 
      University, Shanghai 200032, China. ningyx@fudan.edu.cn
FAU - Ren, Shun-Lin
AU  - Ren SL
FAU - Zhao, Feng-di
AU  - Zhao FD
FAU - Yin, Lian-Hua
AU  - Yin LH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Zhejiang Univ Sci B
JT  - Journal of Zhejiang University. Science. B
JID - 101236535
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Phosphoproteins)
RN  - 0 (steroidogenic acute regulatory protein)
SB  - IM
MH  - ATP-Binding Cassette Transporters/genetics/*metabolism
MH  - Animals
MH  - Cell Line
MH  - Endothelial Cells/*metabolism
MH  - Mice
MH  - Microcirculation/*physiology
MH  - Phosphoproteins/genetics/*metabolism
MH  - Up-Regulation/physiology
PMC - PMC2865837
EDAT- 2010/05/06 06:00
MHDA- 2010/08/24 06:00
PMCR- 2010/05/01
CRDT- 2010/05/06 06:00
PHST- 2010/05/06 06:00 [entrez]
PHST- 2010/05/06 06:00 [pubmed]
PHST- 2010/08/24 06:00 [medline]
PHST- 2010/05/01 00:00 [pmc-release]
AID - 10.1631/jzus.B0900369 [doi]
PST - ppublish
SO  - J Zhejiang Univ Sci B. 2010 May;11(5):350-6. doi: 10.1631/jzus.B0900369.