PMID- 20443864
OWN - NLM
STAT- MEDLINE
DCOM- 20100730
LR  - 20240324
IS  - 1752-8062 (Electronic)
IS  - 1752-8054 (Print)
IS  - 1752-8054 (Linking)
VI  - 2
IP  - 1
DP  - 2009 Feb
TI  - c-Kit+ bone marrow stem cells differentiate into functional cardiac myocytes.
PG  - 26-32
LID - 10.1111/j.1752-8062.2008.00089.x [doi]
AB  - The utility of bone marrow cells (BMCs) to regenerate cardiac myocytes is 
      controversial. The present study examined the capacity of different types of BMCs 
      to generate functional cardiac myocytes. Isolated c-kit(+) BMCs (BMSCs), c-kit(+) 
      and crude BMCs from the adult feline femur were membrane stained with PKH26 dye 
      or infected with a control enhanced green fluorescence protein transcript 
      (EGFP)-adenovirus prior to co-culture upon neonatal rat ventricular myocytes 
      (NRVM). Co-cultured cells were immuno-stained for c-kit, alpha-tropomyosin, 
      alpha-actinin, connexin 43 (Cx43) and Ki67 and analyzed with confocal microscopy. 
      Electrophysiology of BMSC derived myocytes were compared to NRVMs within the same 
      culture dish. Gap junction function was analyzed by fluorescence recovery after 
      photo-bleaching (FRAP). BMCs proliferated and differentiated into cardiac 
      myocytes during the first 48 hours of co-culturing. These newly formed cardiac 
      myocytes were able to contract spontaneously or synchronously with neighboring 
      NRVMs. The myogenic rate of c-kit(+) BMSCs was significantly greater than 
      c-kit(+) and crude BMCs (41.2 +/- 2.1, 6.1 +/- 1.2, and 17.1 +/- 1.5%, 
      respectively). The newly formed cardiac myocytes exhibited an immature 
      electrophysiological phenotype until they became electrically coupled to NRVMs 
      through functional gap junctions. BMSCs did not become functional myocytes in the 
      absence of NRVMs. In conclusion, c-kit(+) BMSCs have the ability to 
      transdifferentiate into functional cardiac myocytes.
FAU - Kubo, Hajime
AU  - Kubo H
AD  - Department of Physiology, Cardiovascular Research Center, Temple University 
      School of Medicine, Philadelphia, PA, USA.
FAU - Berretta, Remus M
AU  - Berretta RM
FAU - Jaleel, Naser
AU  - Jaleel N
FAU - Angert, David
AU  - Angert D
FAU - Houser, Steven R
AU  - Houser SR
LA  - eng
GR  - P01 HL108806/HL/NHLBI NIH HHS/United States
GR  - R01 HL033921/HL/NHLBI NIH HHS/United States
GR  - R0133921/PHS HHS/United States
GR  - R01 HL089312/HL/NHLBI NIH HHS/United States
GR  - P01 HL091799/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Clin Transl Sci
JT  - Clinical and translational science
JID - 101474067
RN  - 0 (Contractile Proteins)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Bone Marrow Cells/*cytology
MH  - Cats
MH  - Cell Communication
MH  - Cell Cycle
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Contractile Proteins/metabolism
MH  - Electrophysiological Phenomena
MH  - Fluorescence Recovery After Photobleaching
MH  - Gap Junctions/metabolism
MH  - Heart Ventricles/cytology
MH  - Myocardial Contraction/physiology
MH  - Myocytes, Cardiac/*cytology/metabolism
MH  - Proto-Oncogene Proteins c-kit/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sarcomeres/metabolism
MH  - Stem Cells/*cytology/*metabolism
PMC - PMC4122301
MID - NIHMS446732
EDAT- 2009/02/01 00:00
MHDA- 2010/07/31 06:00
PMCR- 2009/02/01
CRDT- 2010/05/07 06:00
PHST- 2010/05/07 06:00 [entrez]
PHST- 2009/02/01 00:00 [pubmed]
PHST- 2010/07/31 06:00 [medline]
PHST- 2009/02/01 00:00 [pmc-release]
AID - CTS089 [pii]
AID - 10.1111/j.1752-8062.2008.00089.x [doi]
PST - ppublish
SO  - Clin Transl Sci. 2009 Feb;2(1):26-32. doi: 10.1111/j.1752-8062.2008.00089.x.