PMID- 20446811
OWN - NLM
STAT- MEDLINE
DCOM- 20110415
LR  - 20181201
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 20
IP  - 1
DP  - 2011 Jan
TI  - Hepatocyte growth factor modification promotes the amelioration effects of human 
      umbilical cord mesenchymal stem cells on rat acute kidney injury.
PG  - 103-13
LID - 10.1089/scd.2009.0495 [doi]
AB  - Human umbilical cord-derived mesenchymal stem cells (hucMSCs) are particularly 
      attractive cells for cellular and gene therapy in acute kidney injury (AKI). 
      Adenovirus-mediated gene therapy has been limited by immune reaction and target 
      genes selection. However, in the present study, we investigated the therapeutic 
      effects of hepatocyte growth factor modified hucMSCs (HGF-hucMSCs) in 
      ischemia/reperfusion-induced AKI rat models. In vivo animal models were generated 
      by subjecting to 60 min of bilateral renal injury by clamping the renal pedicles 
      and then introduced HGF-hucMSCs via the left carotid artery. Our results revealed 
      that serum creatinine and urea nitrogen levels decreased to the baseline more 
      quickly in HGF-hucMSCs-treated group than that in hucMSCs- or green fluorescent 
      protein-hucMSCs-treated groups at 72 h after injury. The percent of proliferating 
      cell nuclear antigen-positive cells in HGF-hucMSCs-treated group was higher than 
      that in the hucMSCs or green fluorescent protein-hucMSCs-treated groups. 
      Moreover, injured renal tissues treated with HGF-hucMSCs also exhibited less 
      hyperemia and renal tubule cast during the recovery process. Immunohistochemistry 
      and living body imaging confirmed that HGF-hucMSCs localize to areas of renal 
      injury. Real-time polymerase chain reaction result showed that HGF-hucMSCs also 
      inhibited caspase-3 and interleukin-1beta mRNA expression in injured renal tissues. 
      Western blot also showed HGF-hucMSCs-treated groups had lower expression of 
      interleukin-1beta. Terminal deoxynucleotidyl transferase biotin-deoxyuridine 
      triphosphate (dUTP) nick end labeling method indicated that HGF-hucMSCs-treated 
      group had the least apoptosis cells. In conclusion, our findings suggest that HGF 
      modification promotes the amelioration of ischemia/reperfusion-induced rat renal 
      injury via antiapoptotic and antiinflammatory mechanisms; thus, providing a novel 
      therapeutic application for hucMSCs in AKI.
FAU - Chen, Yuan
AU  - Chen Y
AD  - Jiangsu University, Zhenjiang, People's Republic of China.
FAU - Qian, Hui
AU  - Qian H
FAU - Zhu, Wei
AU  - Zhu W
FAU - Zhang, Xu
AU  - Zhang X
FAU - Yan, Yongmin
AU  - Yan Y
FAU - Ye, Shengqin
AU  - Ye S
FAU - Peng, Xiujuan
AU  - Peng X
FAU - Li, Wei
AU  - Li W
FAU - Xu, Wenrong
AU  - Xu W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101012
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Acute Kidney Injury/complications/physiopathology/*therapy
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Apoptosis
MH  - Cell Cycle
MH  - Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation
MH  - Genetic Vectors
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Inflammation/complications/pathology
MH  - Kidney/pathology
MH  - Kidney Function Tests
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Neoplasms/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/complications/physiopathology
MH  - Transduction, Genetic
MH  - Umbilical Cord/*cytology
EDAT- 2010/05/08 06:00
MHDA- 2011/04/19 06:00
CRDT- 2010/05/08 06:00
PHST- 2010/05/08 06:00 [entrez]
PHST- 2010/05/08 06:00 [pubmed]
PHST- 2011/04/19 06:00 [medline]
AID - 10.1089/scd.2009.0495 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2011 Jan;20(1):103-13. doi: 10.1089/scd.2009.0495. Epub 2010 Oct 
      12.