PMID- 20447769
OWN - NLM
STAT- MEDLINE
DCOM- 20101203
LR  - 20100806
IS  - 1873-2550 (Electronic)
IS  - 0304-4017 (Linking)
VI  - 172
IP  - 1-2
DP  - 2010 Aug 27
TI  - Fluorescence in situ hybridization for identification of Tritrichomonas foetus in 
      formalin-fixed and paraffin-embedded histological specimens of intestinal 
      trichomoniasis.
PG  - 139-43
LID - 10.1016/j.vetpar.2010.04.014 [doi]
AB  - In the present study a highly species-specific oligonucleotide sequence of 
      Tritrichomonas foetus 18S rRNA was used to design an antisense probe for 
      identification of T. foetus in formalin-fixed and paraffin-embedded histological 
      specimens by means of fluorescence in situ hybridization (FISH). Using archival 
      histological specimens from several species with light microscopic evidence of 
      intestinal trichomoniasis, and under optimized hybridization conditions, the 
      probe positively identified trichomonads in colonic specimens from piglets and a 
      kitten with PCR-confirmed T. foetus infection. Neither positive hybridization of 
      the probe or PCR amplification of T. foetus DNA was observed in histological 
      specimens from hamster (Tritrichomonas muris), turkey, nor mouse (Entamoeba 
      muris) intestinal protozoal infections. Sequence-specific binding of the probe 
      was further verified by successfully out-competing the hybridization with 10 x 
      molar excess unlabeled probe and failure of a labeled sense probe to hybridize. 
      The FISH assay described here enables simultaneous location and molecular 
      identification of T. foetus in formalin-fixed and paraffin-embedded histological 
      specimens of intestinal trichomoniasis. The methods employed are likely to also 
      be applicable to probes designed for specific recognition of other trichomonad 
      species, especially in mammalian tissue where red blood cell auto-fluorescence 
      can be easily differentiated from the hybridization signal of trichomonads.
CI  - (c) 2010 Elsevier B.V. All rights reserved.
FAU - Gookin, J L
AU  - Gookin JL
AD  - Department of Clinical Sciences, College of Veterinary Medicine, North Carolina 
      State University, Raleigh, NC, USA. Jody Gookin@ncsu.edu
FAU - Stone, M R
AU  - Stone MR
FAU - Yaeger, M J
AU  - Yaeger MJ
FAU - Meyerholz, D K
AU  - Meyerholz DK
FAU - Moisan, Peter
AU  - Moisan P
LA  - eng
PT  - Journal Article
DEP - 20100418
PL  - Netherlands
TA  - Vet Parasitol
JT  - Veterinary parasitology
JID - 7602745
RN  - 0 (DNA, Protozoan)
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Animals
MH  - Cats
MH  - DNA, Protozoan/chemistry/genetics
MH  - Histocytochemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Intestinal Diseases/diagnosis/*parasitology
MH  - Microscopy, Fluorescence
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 18S/chemistry/genetics
MH  - Swine
MH  - Trichomonas Infections/*diagnosis
MH  - Tritrichomonas foetus/genetics/*isolation & purification
EDAT- 2010/05/08 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/05/08 06:00
PHST- 2009/10/14 00:00 [received]
PHST- 2010/03/23 00:00 [revised]
PHST- 2010/04/09 00:00 [accepted]
PHST- 2010/05/08 06:00 [entrez]
PHST- 2010/05/08 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S0304-4017(10)00233-5 [pii]
AID - 10.1016/j.vetpar.2010.04.014 [doi]
PST - ppublish
SO  - Vet Parasitol. 2010 Aug 27;172(1-2):139-43. doi: 10.1016/j.vetpar.2010.04.014. 
      Epub 2010 Apr 18.