PMID- 20448242
OWN - NLM
STAT- MEDLINE
DCOM- 20111230
LR  - 20200109
IS  - 1718-4304 (Electronic)
IS  - 0896-8608 (Linking)
VI  - 31
IP  - 1
DP  - 2011 Jan-Feb
TI  - Impairment of MCP-1 expression in mesothelial cells exposed to peritoneal 
      dialysis fluid by osmotic stress and acidic stress.
PG  - 80-9
LID - 10.3747/pdi.2009.00159 [doi]
AB  - BACKGROUND: Bacterial peritonitis is one of the most frequent complications in 
      patients on peritoneal dialysis. In the present study, we investigated effects of 
      peritoneal dialysis fluid (PDF) on mesothelial cell recruitment of macrophages, 
      focusing especially on unphysiological properties of PDF. METHODS: Human and 
      murine mesothelial cells were exposed to PDF or individual properties of PDF (low 
      pH, high glucose concentration, hyperosmolality, high lactate concentration) in 
      vitro and in vivo, treated with inflammatory stimuli, and subjected to analyses 
      of monocyte chemoattractant protein-1 (MCP-1), nuclear factor-kappaB (NF-kappaB), 
      mitogen-activated protein (MAP) kinases, and MAP kinase phosphatase-1 (MKP-1). 
      RESULTS: We found that intraperitoneal administration of PDF suppressed 
      expression of MCP-1 and infiltration of mononuclear cells in the peritoneum of 
      mice following injection with lipopolysaccharide. Among the unphysiological 
      properties of PDF, low pH and hyperosmolality caused blunted induction of MCP-1 
      in cytokine-stimulated mesothelial cells. The attenuated response was ascribed to 
      suppression of NF-kappaB by low pH and inhibition of p38 MAP kinase by 
      hyperosmolality. Furthermore, the attenuated phosphorylation of p38 MAP kinase by 
      osmotic stress was associated with induction of MKP-1. CONCLUSION: These results 
      suggest a possibility that mesothelial cells exposed to PDF exhibit attenuated 
      MCP-1 expression and consequent impairment of macrophage recruitment through dual 
      mechanisms, that is, inhibition of NF-kappaB by acidic stress and blunted activation 
      of p38 MAP kinase by osmotic stress. In patients on peritoneal dialysis, blunted 
      expression of chemokines may lead to perturbation of bacterial clearance by 
      macrophages in the peritoneal cavity.
FAU - Ogata, Ryouji
AU  - Ogata R
AD  - Department of Molecular Signaling, Interdisciplinary Graduate School of Medicine 
      and Engineering, University of Yamanashi, Chuo, Yamanashi, Japan.
FAU - Hiramatsu, Nobuhiko
AU  - Hiramatsu N
FAU - Hayakawa, Kunihiro
AU  - Hayakawa K
FAU - Nakajima, Shotaro
AU  - Nakajima S
FAU - Yao, Jian
AU  - Yao J
FAU - Kobayashi, Tetsuro
AU  - Kobayashi T
FAU - Kitamura, Masanori
AU  - Kitamura M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100506
PL  - United States
TA  - Perit Dial Int
JT  - Peritoneal dialysis international : journal of the International Society for 
      Peritoneal Dialysis
JID - 8904033
RN  - 0 (Chemokine CCL2)
RN  - 0 (Dialysis Solutions)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/*drug effects
MH  - Dialysis Solutions/*pharmacology
MH  - Epithelial Cells/*drug effects
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Mice
MH  - Monocytes/drug effects/physiology
MH  - Osmosis
MH  - Stress, Physiological
EDAT- 2010/05/08 06:00
MHDA- 2011/12/31 06:00
CRDT- 2010/05/08 06:00
PHST- 2010/05/08 06:00 [entrez]
PHST- 2010/05/08 06:00 [pubmed]
PHST- 2011/12/31 06:00 [medline]
AID - pdi.2009.00159 [pii]
AID - 10.3747/pdi.2009.00159 [doi]
PST - ppublish
SO  - Perit Dial Int. 2011 Jan-Feb;31(1):80-9. doi: 10.3747/pdi.2009.00159. Epub 2010 
      May 6.