PMID- 20450073
OWN - NLM
STAT- MEDLINE
DCOM- 20100526
LR  - 20160120
IS  - 1006-916X (Print)
IS  - 1006-916X (Linking)
VI  - 16
IP  - 1
DP  - 2010 Feb
TI  - [Detection of bacterial meningitis by multiplex polymerase chain reaction].
PG  - 47-51
AB  - OBJECTIVE: To develop a rapid method for detecting 8 pathogens which were highly 
      related to bacterial meningitis by multiplex polymerase chain reaction. METHOD: 
      By optimizing the reaction condition and amplification program of single pair 
      polymerase chain reaction, the multiplex pairs polymerase chain reactions (M-PCR) 
      was developed to identify eight pathogens simultaneously including Neisseria 
      Meningitis, Haemophilus Influenzae, Streptococcus Pneumoniae, Cryptococcus 
      Neoformans, Staphylococcus Aureus, Listerisa Monocytogene, Streptococcus Suis and 
      Mycobacterium Tuberculosis. Meanwhile, The sensitivity of M-PCR assay was also 
      studied. RESULTS: M-PCR methods for detecting 8 pathogens which could cause 
      bacterial meningitis have been established. M-PCR showed specific, sensitive and 
      more rapid than conventional culturing method. CONCLUSION: This multiplex 
      polymerase chain reaction method can be used for diagnosis and scanning of 
      suspicious bacterial meningitis cases in order to improve the diagnostic positive 
      rate of bacterial meningitis cases.
FAU - Ren, Hong-Yu
AU  - Ren HY
AD  - State Key Laboratory for Infectious Disease Control and Prevention, National 
      Institute for Communicable Disease Control and Prevention, Chinese Center for 
      Disease Control and Prevention, Beijing 102206, China.
FAU - An, Shu-Yi
AU  - An SY
FAU - Li, Ma-Chao
AU  - Li MC
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Yi Miao He Mian Yi
JT  - Zhongguo yi miao he mian yi
JID - 101545586
SB  - IM
MH  - Bacteria/classification/genetics/isolation & purification
MH  - Humans
MH  - Meningitis, Bacterial/*diagnosis
MH  - Polymerase Chain Reaction/*methods
MH  - Time Factors
EDAT- 2010/05/11 06:00
MHDA- 2010/05/27 06:00
CRDT- 2010/05/11 06:00
PHST- 2010/05/11 06:00 [entrez]
PHST- 2010/05/11 06:00 [pubmed]
PHST- 2010/05/27 06:00 [medline]
PST - ppublish
SO  - Zhongguo Yi Miao He Mian Yi. 2010 Feb;16(1):47-51.