PMID- 20450354
OWN - NLM
STAT- MEDLINE
DCOM- 20100907
LR  - 20101118
IS  - 1943-3670 (Electronic)
IS  - 0022-3492 (Linking)
VI  - 81
IP  - 6
DP  - 2010 Jun
TI  - Periosteum-derived cells as an alternative to bone marrow cells for bone tissue 
      engineering around dental implants. A histomorphometric study in beagle dogs.
PG  - 907-16
LID - 10.1902/jop.2010.090604 [doi]
AB  - BACKGROUND: The aim of this study is to investigate the potential use of 
      periosteum-derived cells (PCs) for tissue engineering in peri-implant defects. 
      METHODS: Bone marrow cells (BMCs) and PCs were harvested from seven adult beagle 
      dogs, cultured in vitro, and phenotypically characterized with regard to their 
      osteogenic properties. The animals were then subjected to teeth extraction, and 3 
      months later, two implant sites were drilled, bone dehiscences created, and 
      dental implants placed. Dehiscences were randomly assigned to one of two groups: 
      PCs (PCs + carrier) and BMCs (BMCs + carrier). After 3 months, the animals were 
      sacrificed and the implants with adjacent hard tissues were processed for 
      undecalcified sections. Bone-to-implant contact, bone fill within the limits of 
      implant threads, and new bone area in a zone lateral to the implant were 
      histometrically obtained. RESULTS: In vitro, phenotypic characterization 
      demonstrated that both cell populations presented osteogenic potential, as 
      identified by the mineral nodule formation and the expression of bone markers. 
      Histometrically, an intergroup analysis showed that both cell-treated defects had 
      similar bone fill within the limits of implant threads and bone-to-implant 
      contact (P >0.05), and although a trend toward higher new bone area values was 
      found for the PC group, there was no significant difference between the 
      experimental groups (P >0.05). CONCLUSIONS: Periosteal and bone marrow cells 
      presented a similar potential for bone reconstruction. As such, periosteum may be 
      considered as an alternative source of osteogenic cells in implant dentistry.
FAU - Ribeiro, Fernanda V
AU  - Ribeiro FV
AD  - Department of Prosthodontics and Periodontics, School of Dentistry at Piracicaba, 
      University of Campinas (UNICAMP), Piracicaba, Sao Paulo, Brazil.
FAU - Suaid, Fabricia F
AU  - Suaid FF
FAU - Ruiz, Karina G S
AU  - Ruiz KG
FAU - Salmon, Cristiane R
AU  - Salmon CR
FAU - Paparotto, Telma
AU  - Paparotto T
FAU - Nociti, Francisco H Jr
AU  - Nociti FH Jr
FAU - Sallum, Enilson A
AU  - Sallum EA
FAU - Casati, Marcio Z
AU  - Casati MZ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Collagen Type I)
RN  - 0 (Integrin-Binding Sialoprotein)
RN  - 0 (Sialoglycoproteins)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/biosynthesis
MH  - Animals
MH  - Bone Marrow Transplantation
MH  - Cell Adhesion
MH  - Cell Proliferation
MH  - *Cell Transplantation
MH  - Cells, Cultured
MH  - Collagen Type I/biosynthesis
MH  - Dental Implantation, Endosseous
MH  - Dogs
MH  - Integrin-Binding Sialoprotein
MH  - *Osseointegration
MH  - *Osteogenesis
MH  - Periosteum/*cytology/metabolism
MH  - Random Allocation
MH  - Sialoglycoproteins/biosynthesis
MH  - Surgical Wound Dehiscence/therapy
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds
EDAT- 2010/05/11 06:00
MHDA- 2010/09/08 06:00
CRDT- 2010/05/11 06:00
PHST- 2010/05/11 06:00 [entrez]
PHST- 2010/05/11 06:00 [pubmed]
PHST- 2010/09/08 06:00 [medline]
AID - 10.1902/jop.2010.090604 [doi]
PST - ppublish
SO  - J Periodontol. 2010 Jun;81(6):907-16. doi: 10.1902/jop.2010.090604.