PMID- 20450986
OWN - NLM
STAT- MEDLINE
DCOM- 20101229
LR  - 20151119
IS  - 1879-3649 (Electronic)
IS  - 1537-1891 (Linking)
VI  - 53
IP  - 3-4
DP  - 2010 Sep-Oct
TI  - Adult rats are more sensitive to the vascular effects induced by 
      hyperhomocysteinemia than young rats.
PG  - 99-106
LID - 10.1016/j.vph.2010.04.006 [doi]
AB  - We aimed to investigate the vascular effects of hyperhomocysteinemia (HHcy) on 
      carotid arteries from young and adult rats. With this purpose young and adult 
      rats received a solution of DL-homocysteine-thiolactone (1 g/kg body weight/day) 
      in the drinking water for 7, 14 and 28 days. Increase on plasma homocysteine 
      occurred in young and adult rats treated with DL-homocysteine-thiolactone in all 
      periods. Vascular reactivity experiments using standard muscle bath procedures 
      showed that HHcy enhanced the contractile response of endothelium-intact, carotid 
      rings to phenylephrine in both young and adult rats. However, in young rats, the 
      increased phenylephrine-induced contraction was observed after 
      hyperhomocysteinemia for 14 and 28 days, whereas in adult rats this response was 
      already apparent after 7 day treatment. HHcy impaired acetylcholine-induced 
      relaxation in arteries from adult but not young rats. The contraction induced by 
      phenylephrine in carotid arteries in the presence of Y-27632 was reversed to 
      control values in arteries from young but not adult rats with 
      hyperhomocysteinemia. HHcy did not alter the contraction induced by CaCl(2) in 
      carotid arteries from young rats, but enhanced CaCl(2)-induced contraction in the 
      arteries from adult rats. HHcy increased the basal levels of superoxide anion in 
      arteries from both groups. Finally, HHcy decreased the basal levels of nitrite in 
      arteries from adult but not young rats. The major new finding of the present work 
      is that arteries from young rats are more resistant to vascular changes evoked by 
      HHcy than arteries from adult rats. Also, we verified that the enhanced vascular 
      response to phenylephrine observed in carotid arteries of DL-homocysteine 
      thiolactone-treated rats is mediated by different mechanisms in young and adult 
      rats.
CI  - Copyright 2010. Published by Elsevier Inc.
FAU - de Andrade, Claudia Roberta
AU  - de Andrade CR
AD  - Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of 
      Sao Paulo (USP), Ribeirao Preto, SP, Brazil.
FAU - de Campos, Glenda Andrea Destro
AU  - de Campos GA
FAU - Tirapelli, Carlos Renato
AU  - Tirapelli CR
FAU - Laurindo, Francisco R M
AU  - Laurindo FR
FAU - Haddad, Renato
AU  - Haddad R
FAU - Eberlin, Marcos N
AU  - Eberlin MN
FAU - de Oliveira, Ana Maria
AU  - de Oliveira AM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20100505
PL  - United States
TA  - Vascul Pharmacol
JT  - Vascular pharmacology
JID - 101130615
RN  - 0 (Amides)
RN  - 0 (Cyclooxygenase Inhibitors)
RN  - 0 (Pyridines)
RN  - 0 (Vasoconstrictor Agents)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 11062-77-4 (Superoxides)
RN  - 138381-45-0 (Y 27632)
RN  - 1WS297W6MV (Phenylephrine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - D5H88XF24X (homocysteine thiolactone)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
RN  - M4I0D6VV5M (Calcium Chloride)
RN  - N9YNS0M02X (Acetylcholine)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Age Factors
MH  - Amides/pharmacology
MH  - Animals
MH  - Calcium Chloride/pharmacology
MH  - Carotid Arteries/*drug effects/physiopathology
MH  - Cyclooxygenase Inhibitors/pharmacology
MH  - Endothelium, Vascular/drug effects/physiopathology
MH  - Homocysteine/*analogs & derivatives/blood/pharmacology
MH  - Hyperhomocysteinemia/*physiopathology
MH  - Indomethacin/pharmacology
MH  - Male
MH  - Muscle Contraction/drug effects
MH  - Muscle, Smooth, Vascular/drug effects/physiopathology
MH  - Nitric Oxide/physiology
MH  - Phenylephrine/pharmacology
MH  - Pyridines/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Superoxides/metabolism
MH  - Vasoconstrictor Agents/pharmacology
MH  - Vasodilation/*drug effects
MH  - rho-Associated Kinases/physiology
EDAT- 2010/05/11 06:00
MHDA- 2010/12/30 06:00
CRDT- 2010/05/11 06:00
PHST- 2009/10/22 00:00 [received]
PHST- 2010/04/19 00:00 [revised]
PHST- 2010/04/22 00:00 [accepted]
PHST- 2010/05/11 06:00 [entrez]
PHST- 2010/05/11 06:00 [pubmed]
PHST- 2010/12/30 06:00 [medline]
AID - S1537-1891(10)00087-X [pii]
AID - 10.1016/j.vph.2010.04.006 [doi]
PST - ppublish
SO  - Vascul Pharmacol. 2010 Sep-Oct;53(3-4):99-106. doi: 10.1016/j.vph.2010.04.006. 
      Epub 2010 May 5.