PMID- 20451412
OWN - NLM
STAT- MEDLINE
DCOM- 20100621
LR  - 20230815
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 32
IP  - 5
DP  - 2010 May 28
TI  - Human immunodeficiency virus-1 inhibition of immunoamphisomes in dendritic cells 
      impairs early innate and adaptive immune responses.
PG  - 654-69
LID - 10.1016/j.immuni.2010.04.011 [doi]
AB  - Dendritic cells (DCs) in mucosal surfaces are early targets for human 
      immunodeficiency virus-1 (HIV-1). DCs mount rapid and robust immune responses 
      upon pathogen encounter. However, immune response in the early events of HIV-1 
      transmission appears limited, suggesting that HIV-1 evade early immune control by 
      DCs. We report that HIV-1 induces a rapid shutdown of autophagy and 
      immunoamphisomes in DCs. HIV-1 envelope activated the mammalian target of 
      rapamycin pathway in DCs, leading to autophagy exhaustion. HIV-1-induced 
      inhibition of autophagy in DC increased cell-associated HIV-1 and transfer of 
      HIV-1 infection to CD4(+) T cells. HIV-1-mediated downregulation of autophagy in 
      DCs impaired innate and adaptive immune responses. Immunoamphisomes in DCs engulf 
      incoming pathogens and appear to amplify pathogen degradation as well as 
      Toll-like receptor responses and antigen presentation. The findings that HIV-1 
      downregulates autophagy and impedes immune functions of DCs represent a 
      pathogenesis mechanism that can be pharmacologically countered with therapeutic 
      and prophylactic implications.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Blanchet, Fabien P
AU  - Blanchet FP
AD  - Department of Dermatology, University Hospital and Medical School of Geneva, 1211 
      Geneva, Switzerland.
FAU - Moris, Arnaud
AU  - Moris A
FAU - Nikolic, Damjan S
AU  - Nikolic DS
FAU - Lehmann, Martin
AU  - Lehmann M
FAU - Cardinaud, Sylvain
AU  - Cardinaud S
FAU - Stalder, Romaine
AU  - Stalder R
FAU - Garcia, Eduardo
AU  - Garcia E
FAU - Dinkins, Christina
AU  - Dinkins C
FAU - Leuba, Florence
AU  - Leuba F
FAU - Wu, Li
AU  - Wu L
FAU - Schwartz, Olivier
AU  - Schwartz O
FAU - Deretic, Vojo
AU  - Deretic V
FAU - Piguet, Vincent
AU  - Piguet V
LA  - eng
GR  - R01 AI042999/AI/NIAID NIH HHS/United States
GR  - R01-AI068493/AI/NIAID NIH HHS/United States
GR  - R01 AI068493-06/AI/NIAID NIH HHS/United States
GR  - R37 AI042999/AI/NIAID NIH HHS/United States
GR  - R01 AI068493/AI/NIAID NIH HHS/United States
GR  - T32 AI007538/AI/NIAID NIH HHS/United States
GR  - R01 AI068493-05/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100506
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (ribosomal protein S6 kinase, 70kD, polypeptide 1)
SB  - IM
CIN - Immunity. 2010 May 28;32(5):587-90. PMID: 20510868
MH  - *Adaptive Immunity
MH  - Autophagy
MH  - Base Sequence
MH  - CD4-Positive T-Lymphocytes/virology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/pathology/*virology
MH  - Down-Regulation
MH  - Flow Cytometry
MH  - HIV Infections/*immunology
MH  - HIV-1/*physiology
MH  - Humans
MH  - *Immunity, Innate
MH  - Immunoblotting
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Lysosomes/immunology/virology
MH  - Molecular Sequence Data
MH  - Phagosomes/*immunology/virology
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases
PMC - PMC2929482
MID - NIHMS205642
EDAT- 2010/05/11 06:00
MHDA- 2010/06/22 06:00
PMCR- 2011/05/28
CRDT- 2010/05/11 06:00
PHST- 2009/07/09 00:00 [received]
PHST- 2009/12/10 00:00 [revised]
PHST- 2010/03/23 00:00 [accepted]
PHST- 2010/05/11 06:00 [entrez]
PHST- 2010/05/11 06:00 [pubmed]
PHST- 2010/06/22 06:00 [medline]
PHST- 2011/05/28 00:00 [pmc-release]
AID - S1074-7613(10)00160-3 [pii]
AID - 10.1016/j.immuni.2010.04.011 [doi]
PST - ppublish
SO  - Immunity. 2010 May 28;32(5):654-69. doi: 10.1016/j.immuni.2010.04.011. Epub 2010 
      May 6.