PMID- 20451511
OWN - NLM
STAT- MEDLINE
DCOM- 20101008
LR  - 20131121
IS  - 1873-2933 (Electronic)
IS  - 0009-9120 (Linking)
VI  - 43
IP  - 12
DP  - 2010 Aug
TI  - Elaidic acid sustains LPS and TNF-alpha induced ICAM-1 and VCAM-I expression on 
      human bone marrow endothelial cells (HBMEC).
PG  - 968-72
LID - 10.1016/j.clinbiochem.2010.04.066 [doi]
AB  - OBJECTIVES: Elaidic acid, the predominant trans-fatty acid in industrially 
      hydrogenated oils, exists on high levels in Iranian hydrogenated oils and 
      margarines. This study was undertaken to investigate the effect of elaidic acid 
      and its cis-counterpart oleic acid on expression of ICAM-1 and VCAM-1 on human 
      bone marrow endothelial cells (HBMECs). DESIGN AND METHODS: HBMEC were 
      pre-treated with TNF-alpha or LPS for induction of the adhesion molecules 
      expression, and then treated with elaidic acid or oleic acid. Soluble and cell 
      associated forms of ICAM-1 and VCAM-1 were quantified by ELISA and Western blot. 
      RESULTS: Our findings indicated that oleic acid suppresses VCAM-1 and ICAM-1 
      expression on HBMEC near to the basal level. Conversely, elaidic acid maintained 
      the level of VCAM-1 and ICAM-1 up-regulated by TNF-alpha or LPS. CONCLUSIONS: It 
      is suggested that elaidic acid could keep the HBMEC at the stimulated phenotype. 
      These findings provide further support on the detrimental effects of elaidic acid 
      in promotion and induction of cardiovascular diseases (CVD).
CI  - 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All 
      rights reserved.
FAU - Sanadgol, Nima
AU  - Sanadgol N
AD  - Department of Biology, College of Science, Zabol University, Zabol, Iran.
FAU - Mostafaie, Ali
AU  - Mostafaie A
FAU - Bahrami, Gholamreza
AU  - Bahrami G
FAU - Mansouri, Kamran
AU  - Mansouri K
FAU - Ghanbari, Fariba
AU  - Ghanbari F
FAU - Bidmeshkipour, Ali
AU  - Bidmeshkipour A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100506
PL  - United States
TA  - Clin Biochem
JT  - Clinical biochemistry
JID - 0133660
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oleic Acids)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 2UMI9U37CP (Oleic Acid)
RN  - 4837010H8C (elaidic acid)
SB  - IM
MH  - Blotting, Western
MH  - Bone Marrow Cells/*cytology
MH  - Cells, Cultured
MH  - Endothelial Cells/cytology/*drug effects/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/*metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Oleic Acid/*pharmacology
MH  - Oleic Acids/pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Vascular Cell Adhesion Molecule-1/*metabolism
EDAT- 2010/05/11 06:00
MHDA- 2010/10/12 06:00
CRDT- 2010/05/11 06:00
PHST- 2009/11/19 00:00 [received]
PHST- 2010/04/08 00:00 [revised]
PHST- 2010/04/20 00:00 [accepted]
PHST- 2010/05/11 06:00 [entrez]
PHST- 2010/05/11 06:00 [pubmed]
PHST- 2010/10/12 06:00 [medline]
AID - S0009-9120(10)00199-2 [pii]
AID - 10.1016/j.clinbiochem.2010.04.066 [doi]
PST - ppublish
SO  - Clin Biochem. 2010 Aug;43(12):968-72. doi: 10.1016/j.clinbiochem.2010.04.066. 
      Epub 2010 May 6.