PMID- 20452684
OWN - NLM
STAT- MEDLINE
DCOM- 20101208
LR  - 20100805
IS  - 1872-8464 (Electronic)
IS  - 0165-5876 (Linking)
VI  - 74
IP  - 7
DP  - 2010 Jul
TI  - Helicobacter pylori colonization of the adenotonsillar tissue: fact or fiction?
PG  - 807-11
LID - 10.1016/j.ijporl.2010.04.007 [doi]
AB  - OBJECTIVE: The transmission of the gastric pathogen Helicobacter pylori involves 
      the oral route. Molecular techniques have allowed the detection of H. pylori DNA 
      in samples of the oral cavity, although culture of H. pylori from these type of 
      samples has been sporadic. Studies have tried to demonstrate the presence of H. 
      pylori in adenotonsillar tissue, with contradictory results. Our aim was to 
      clarify whether the adenotonsillar tissue may constitute an extra gastric 
      reservoir for H. pylori. METHODS: Sixty-two children proposed for adenoidectomy 
      or tonsillectomy were enrolled. A total of 101 surgical specimens, 55 adenoid and 
      46 tonsils, were obtained. Patients were characterized for the presence of 
      anti-H. pylori antibodies by serology. On each surgical sample rapid urease test, 
      immunohistochemistry, fluorescence in situ hybridization (FISH) with a peptide 
      nucleic acid probe for H. pylori, and polymerase chain reaction-DNA hybridization 
      assay (PCR-DEIA) directed to the vacA gene of H. pylori were performed. RESULTS: 
      Thirty-nine percent of the individuals had anti-H. pylori antibodies. Rapid 
      urease test was positive in samples of three patients, all with positive 
      serology. Immunohistochemistry was positive in samples of two patients, all with 
      negative serology. All rapid urease test or immunohistochemistry positive cases 
      were negative by FISH. All samples tested were negative when PCR-DEIA for H. 
      pylori detection was used directly in adenotonsillar specimens. CONCLUSIONS: The 
      adenotonsillar tissue does not constitute an extra gastric reservoir for H. 
      pylori infection, at least a permanent one, in this population of children. 
      Moreover, techniques currently used for detecting gastric H. pylori colonization 
      are not adequate to evaluate infection of the adenotonsillar tissues.
CI  - Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
FAU - Vilarinho, Sergio
AU  - Vilarinho S
AD  - Servico de Otorrinolaringologia, Hospital de Sao Marcos, Braga, Portugal.
FAU - Guimaraes, Nuno M
AU  - Guimaraes NM
FAU - Ferreira, Rui M
AU  - Ferreira RM
FAU - Gomes, Barbara
AU  - Gomes B
FAU - Wen, Xiaogang
AU  - Wen X
FAU - Vieira, Maria J
AU  - Vieira MJ
FAU - Carneiro, Fatima
AU  - Carneiro F
FAU - Godinho, Tiago
AU  - Godinho T
FAU - Figueiredo, Ceu
AU  - Figueiredo C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100510
PL  - Ireland
TA  - Int J Pediatr Otorhinolaryngol
JT  - International journal of pediatric otorhinolaryngology
JID - 8003603
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Nucleic Acid Probes)
RN  - EC 3.5.1.5 (Urease)
SB  - IM
MH  - Adenoidectomy
MH  - Adenoids/*microbiology
MH  - Antibodies, Bacterial/blood
MH  - Child
MH  - DNA, Bacterial/genetics
MH  - Female
MH  - Helicobacter Infections/*diagnosis
MH  - Helicobacter pylori/immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Nucleic Acid Probes
MH  - Palatine Tonsil/*microbiology
MH  - Polymerase Chain Reaction
MH  - Tonsillectomy
MH  - Urease/analysis
EDAT- 2010/05/11 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/05/11 06:00
PHST- 2010/03/23 00:00 [received]
PHST- 2010/04/13 00:00 [revised]
PHST- 2010/04/14 00:00 [accepted]
PHST- 2010/05/11 06:00 [entrez]
PHST- 2010/05/11 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S0165-5876(10)00180-1 [pii]
AID - 10.1016/j.ijporl.2010.04.007 [doi]
PST - ppublish
SO  - Int J Pediatr Otorhinolaryngol. 2010 Jul;74(7):807-11. doi: 
      10.1016/j.ijporl.2010.04.007. Epub 2010 May 10.