PMID- 20454664
OWN - NLM
STAT- MEDLINE
DCOM- 20100907
LR  - 20211203
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 5
IP  - 5
DP  - 2010 May 3
TI  - Platelet factor 4 regulation of monocyte KLF4 in experimental cerebral malaria.
PG  - e10413
LID - 10.1371/journal.pone.0010413 [doi]
LID - e10413
AB  - Cerebral malaria continues to be a difficult to treat complication of Plasmodium 
      falciparum infection in children. We have shown that platelets can have major 
      deleterious immune functions in experimental cerebral malaria (ECM). One of the 
      platelet derived mediators we have identified as particularly important is 
      platelet factor 4/CXCL4. Our prior work demonstrated that PF4(-/-) mice are 
      protected from ECM, have reduced plasma cytokines, and have reduced T-cell 
      trafficking to the brain. We now show that PF4 drives monocyte cytokine 
      production in a Kruppel like factor 4 (KLF4) dependent manner. Monocyte depleted 
      Plasmodium berghei infected mice have improved survival, and KLF4 is greatly 
      increased in control, but not monocyte depleted mice. PF4(-/-) mice have less 
      cerebral monocyte trafficking and no change in KLF4 expression. These data 
      indicate that PF4 induction of monocyte KLF4 expression may be an important step 
      in the pathogenesis of ECM.
FAU - Srivastava, Kalyan
AU  - Srivastava K
AD  - Aab Cardiovascular Research Institute, University of Rochester School of Medicine 
      and Dentistry, Rochester, New York, United States of America.
FAU - Field, David J
AU  - Field DJ
FAU - Aggrey, Angela
AU  - Aggrey A
FAU - Yamakuchi, Munekazu
AU  - Yamakuchi M
FAU - Morrell, Craig N
AU  - Morrell CN
LA  - eng
GR  - R01 HL093179/HL/NHLBI NIH HHS/United States
GR  - R01 HL094547/HL/NHLBI NIH HHS/United States
GR  - R01HL093179/HL/NHLBI NIH HHS/United States
GR  - R01HL094547/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100503
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cytokines)
RN  - 0 (KLF4 protein, human)
RN  - 0 (Klf4 protein, mouse)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 37270-94-3 (Platelet Factor 4)
SB  - IM
MH  - Animals
MH  - Cytokines/biosynthesis
MH  - Humans
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors/*metabolism
MH  - Malaria, Cerebral/*metabolism/*pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monocytes/*metabolism
MH  - Platelet Factor 4/*metabolism
PMC - PMC2862712
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2010/05/11 06:00
MHDA- 2010/09/08 06:00
PMCR- 2010/05/03
CRDT- 2010/05/11 06:00
PHST- 2009/12/17 00:00 [received]
PHST- 2010/04/08 00:00 [accepted]
PHST- 2010/05/11 06:00 [entrez]
PHST- 2010/05/11 06:00 [pubmed]
PHST- 2010/09/08 06:00 [medline]
PHST- 2010/05/03 00:00 [pmc-release]
AID - 09-PONE-RA-14991R1 [pii]
AID - 10.1371/journal.pone.0010413 [doi]
PST - epublish
SO  - PLoS One. 2010 May 3;5(5):e10413. doi: 10.1371/journal.pone.0010413.