PMID- 20458160
OWN - NLM
STAT- MEDLINE
DCOM- 20110121
LR  - 20211020
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 11
IP  - 2
DP  - 2010 Jun
TI  - Development and characterization of stable cell lines constitutively expressing 
      the porcine reproductive and respiratory syndrome virus nucleocapsid protein.
PG  - 169-71
AB  - Despite global efforts to control porcine reproductive and respiratory syndrome 
      virus (PRRSV) infection, the virus continues to cause economic problems in the 
      swine industry worldwide. In this study, we attempted to generate and 
      characterize a panel of stable BHK cell lines that constitutively express the 
      nucleocapsid (N) protein of type 1 or type 2 PRRSV. The established BHK cell 
      lines were found to react well with N-specific antibodies as well as the 
      hyperimmune serum of pigs raised against each genotype of PRRSV. Taken together, 
      the data implicate a potential usefulness for the newly generated stable cell 
      lines as a diagnostic reagent for PRRSV serology.
FAU - Sagong, Mingeun
AU  - Sagong M
AD  - Department of Microbiology, College of Natural Sciences, Kyungpook National 
      University, Daegu 702-701, Korea.
FAU - Park, Choi Kyu
AU  - Park CK
FAU - Kim, Seong Hee
AU  - Kim SH
FAU - Moon, Sung Up
AU  - Moon SU
FAU - Cho, Seong Cheol
AU  - Cho SC
FAU - Lee, Changhee
AU  - Lee C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Antibodies, Viral)
RN  - 0 (Nucleocapsid Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/analysis/immunology
MH  - Blotting, Western/veterinary
MH  - Cell Line
MH  - Cricetinae
MH  - Female
MH  - Genotype
MH  - Nucleocapsid Proteins/genetics/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine
MH  - Transfection/veterinary
PMC - PMC2873819
EDAT- 2010/05/12 06:00
MHDA- 2011/01/22 06:00
PMCR- 2010/06/01
CRDT- 2010/05/12 06:00
PHST- 2010/05/12 06:00 [entrez]
PHST- 2010/05/12 06:00 [pubmed]
PHST- 2011/01/22 06:00 [medline]
PHST- 2010/06/01 00:00 [pmc-release]
AID - 201006169 [pii]
AID - 10.4142/jvs.2010.11.2.169 [doi]
PST - ppublish
SO  - J Vet Sci. 2010 Jun;11(2):169-71. doi: 10.4142/jvs.2010.11.2.169.