PMID- 20458916
OWN - NLM
STAT- MEDLINE
DCOM- 20111205
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 28
IP  - 1
DP  - 2010 Jan
TI  - [Rapid simultaneous determination of 42 psychoactive drugs and their metabolites 
      in human plasma and urine by ultra performance liquid chromatography-tandem mass 
      spectrometry].
PG  - 23-33
AB  - A rapid method for the simultaneous determination of 42 psychoactive drugs and 
      their metabolites (barbitals, benzodiazepines, tricyclic antidepressants, 
      phenothiazines, etc. ) in human plasma and urine was developed using ultra 
      performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After a 
      simple protein precipitation step, the analysis of the drugs and the metabolites 
      was achieved on an Acquity UPLC BEH C18 column by using the gradient elution with 
      ammonium acetate and methanol-acetonitrile (1: 1, v/v) as the mobile phases, and 
      detected by electrospray ionization-tandem mass spectrometry in the multiple 
      reaction monitoring (MRM) mode operated simultaneously in both positive and 
      negative modes by rapid switching, and quantified by matrix-match standard 
      solution. The average recoveries were 60.2% - 125% and 64.5% - 126% for the drugs 
      in plasma and urine except those of perphenazine, thioridazine and 
      chloropromazine in plasma were 37.6% - 57.5%, 36.3% - 48.3%, 52.4% -67.4%, 
      respectively; trazodone and diazepam in urine were 100% -142% and 108% - 177%, 
      respectively. The relative standard deviations (RSDs) of all the drugs in plasma 
      and urine were within 0.8% - 26% and 2.6%18% (n = 6), respectively. The detection 
      limits of the 4 barbitals ranged from 20 to 100 mg/L and those of the other drugs 
      ranged from 0.05 to 2.0 mg/L. The method is simple, selective and sensitive to 
      detect drugs for both clinical and forensic purposes.
FAU - Zhang, Xiuyao
AU  - Zhang X
AD  - Wenzhou Center for Disease Control and Prevention, Wenzhou 325001, China. 
      xyzwz123@126.com
FAU - Cai, Xinxin
AU  - Cai X
FAU - Zhang, Xiaoyi
AU  - Zhang X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Antidepressive Agents, Tricyclic)
RN  - 0 (Psychotropic Drugs)
RN  - 12794-10-4 (Benzodiazepines)
RN  - 5WZ53ENE2P (Barbital)
SB  - IM
MH  - Antidepressive Agents, Tricyclic/blood/urine
MH  - Barbital/blood/urine
MH  - Benzodiazepines/blood/urine
MH  - Blood Chemical Analysis/methods
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Psychotropic Drugs/*blood/metabolism/*urine
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2010/05/13 06:00
MHDA- 2011/12/13 00:00
CRDT- 2010/05/13 06:00
PHST- 2010/05/13 06:00 [entrez]
PHST- 2010/05/13 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.3724/sp.j.1123.2010.00023 [doi]
PST - ppublish
SO  - Se Pu. 2010 Jan;28(1):23-33. doi: 10.3724/sp.j.1123.2010.00023.