PMID- 20458923
OWN - NLM
STAT- MEDLINE
DCOM- 20111205
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 28
IP  - 1
DP  - 2010 Jan
TI  - [Simultaneous determination of five effective components in Sijunzi bolus using 
      high performance liquid chromatography-evaporation light scattering detection].
PG  - 64-7
AB  - A high performance liquid chromatographic (HPLC) method was developed for the 
      simultaneous determination of lobetyolin, pachymic acid, glycyrrhizic acid, 
      atractylenoide III and atractylenolide I in Sijunzi bolus. The separation was 
      performed on an HIQ SIL C18 V column (250 mm x 4.6 mm, 5 microm) with 0.5% acetic 
      acid-methanol as the mobile phase of gradient elution at a flow rate of 1.0 
      mL/min. The detection was performed with an evaporation light scattering detector 
      (ELSD) and the sample volume was 10 microL. The temperature of drift tube and 
      heating grade of nebulizer was respectively set at 55 degrees C and 60% at 0.2 
      MPa of pressure. Nitrogen gas was used as carrier gas. Under the optimized 
      conditions, there were good linear relationships between the logarithm values of 
      mass concentration and the peak areas of lobetyolin, pachymic acid, glycyrrhizic 
      acid, atractylenoide III and atractylenolide I in the ranges of 0.076 - 1.21, 
      0.048 -0.76, 0.153 - 2.45, 0.045 - 0.72 and 0.098 - 1.56 g/L, respectively. The 
      recoveries of the five components were between 97.13% and 100.25%, the relative 
      standard deviations (RSDs) were between 1.23% and 2.44%. This method is simple, 
      rapid, accurate and suitable for the quality control of Sijunzi bolus.
FAU - Li, Chunying
AU  - Li C
AD  - Graduate School of Northeast Forestry University, Harbin 150040, China. 
      nefulcy@163.com
FAU - Zhang, Xiaojun
AU  - Zhang X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drugs, Chinese Herbal/analysis/*chemistry
MH  - *Light
MH  - Limit of Detection
MH  - Medicine, Chinese Traditional
MH  - *Scattering, Radiation
EDAT- 2010/05/13 06:00
MHDA- 2011/12/13 00:00
CRDT- 2010/05/13 06:00
PHST- 2010/05/13 06:00 [entrez]
PHST- 2010/05/13 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.3724/sp.j.1123.2010.00064 [doi]
PST - ppublish
SO  - Se Pu. 2010 Jan;28(1):64-7. doi: 10.3724/sp.j.1123.2010.00064.