PMID- 20459281
OWN - NLM
STAT- MEDLINE
DCOM- 20100913
LR  - 20190930
IS  - 1560-2281 (Electronic)
IS  - 1083-3668 (Linking)
VI  - 15
IP  - 2
DP  - 2010 Mar-Apr
TI  - Monitoring human leukocyte antigen class I molecules by micro-Raman spectroscopy 
      at single-cell level.
PG  - 027007
LID - 10.1117/1.3368687 [doi]
AB  - Human leukocyte antigen (HLA) class I molecules are formed by three 
      immunoglobulin-like domains (alpha1, alpha2, and alpha3) once folded by peptide 
      and beta(2)-microglobulin show the presence of two alpha-helix streams and one 
      beta-sheet limiting the pocket for the antigenic peptide. The loss of HLA class I 
      expression in tumors and virus-infected cells, on one hand, prevents T cell 
      recognition, while on the other hand, it leads to natural killer (NK) cell 
      mediated cytotoxicity. We propose the possibility of using Raman spectroscopy to 
      measure the relative expression of HLA class I molecules at the single-cell 
      level. Raman spectra are recorded for three cell lines (K562, T2, and T3) and 
      monomers (HLA class I folded, unfolded and peptide+beta(2)-microlobulin refolded) 
      using 830 nm laser line. Our data are consistent with the hypothesis that in the 
      Raman spectra, ranging from 1600 to 1800 cm(-1), the intensity variation of cells 
      associated with HLA class I molecules could be measured.
FAU - Das, Gobind
AU  - Das G
AD  - Universita Magna Graecia di Catanzaro, Dipartimento di Medicina Sperimentale e 
      Clinica, Lab BIONEM, Catanzaro, 88100 Italy. gobind@unicz.it
FAU - La Rocca, Rosanna
AU  - La Rocca R
FAU - Lakshmikanth, Tadepally
AU  - Lakshmikanth T
FAU - Gentile, Francesco
AU  - Gentile F
FAU - Tallerico, Rossana
AU  - Tallerico R
FAU - Zambetti, Lia P
AU  - Zambetti LP
FAU - Devitt, J
AU  - Devitt J
FAU - Candeloro, Patrizio
AU  - Candeloro P
FAU - De Angelis, Francesco
AU  - De Angelis F
FAU - Carbone, Ennio
AU  - Carbone E
FAU - Di Fabrizio, Enzo
AU  - Di Fabrizio E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biomed Opt
JT  - Journal of biomedical optics
JID - 9605853
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Cells, Cultured
MH  - Histocompatibility Antigens Class I/*blood
MH  - Humans
MH  - Leukocytes/*immunology
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrum Analysis, Raman/*methods
EDAT- 2010/05/13 06:00
MHDA- 2010/09/14 06:00
CRDT- 2010/05/13 06:00
PHST- 2010/05/13 06:00 [entrez]
PHST- 2010/05/13 06:00 [pubmed]
PHST- 2010/09/14 06:00 [medline]
AID - 10.1117/1.3368687 [doi]
PST - ppublish
SO  - J Biomed Opt. 2010 Mar-Apr;15(2):027007. doi: 10.1117/1.3368687.