PMID- 20459530
OWN - NLM
STAT- MEDLINE
DCOM- 20100813
LR  - 20161125
IS  - 1365-2559 (Electronic)
IS  - 0309-0167 (Linking)
VI  - 56
IP  - 3
DP  - 2010 Feb
TI  - A UK NEQAS ICC and ISH multicentre study using the Kreatech Poseidon HER2 FISH 
      probe: intersite variation can be rigorously controlled using FISH.
PG  - 297-304
LID - 10.1111/j.1365-2559.2010.03493.x [doi]
AB  - AIM: To assess a new HER2 fluorescence in situ hybridization (FISH) test and 
      report on multicentre intrasite and intersite variation. METHODS AND RESULTS: 
      HER2 results were scored from 45 breast cancers in eight laboratories using the 
      Kreatech Poseidon HER2 FISH probe (Kreatech Diagnostics, Amsterdam, the 
      Netherlands). Overall, 80.9% of cores were successfully analysed. Mean intrasite 
      variation for HER2 ratio assessment was low (4.74%). Intersite variation in ratio 
      was in line with previous reports (11.9+/-0.8%) for both reference and 
      non-reference laboratories; only one laboratory displayed significantly higher 
      intersite variation (P=0.009) than the remaining seven laboratories. The overall 
      incidence of misclassification of cores was <1.3%, demonstrating an excellent 
      level of concordance (>98.7%) across all eight laboratories, irrespective of 
      whether they were 'reference' or 'routine diagnostic' laboratories. CONCLUSIONS: 
      The Kreatech Poseidon HER2 FISH test is robust and reproducible. Highly 
      quantitatively reproducible FISH results were obtained from eight 'diagnostic' 
      and 'reference' laboratories; however, continued quality assessments are 
      essential to good performance.
FAU - Bartlett, John M S
AU  - Bartlett JM
AD  - Endocrine Cancer Group, Edinburgh University Cancer Research Centre, Western 
      General Hospital, Edinburgh, UK. john.bartlett@ed.ac.uk
FAU - Campbell, Fiona M
AU  - Campbell FM
FAU - Ibrahim, Merdol
AU  - Ibrahim M
FAU - Thomas, Jeremy
AU  - Thomas J
FAU - Wencyk, Pete
AU  - Wencyk P
FAU - Ellis, Ian
AU  - Ellis I
FAU - Kay, Elaine
AU  - Kay E
FAU - Connolly, Yvonne
AU  - Connolly Y
FAU - O'Grady, Anthony
AU  - O'Grady A
FAU - Barnett, Sarah
AU  - Barnett S
FAU - Starczynski, Jane
AU  - Starczynski J
FAU - Cunningham, Paul
AU  - Cunningham P
FAU - Miller, Keith
AU  - Miller K
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Female
MH  - Gene Expression Profiling/methods/*standards
MH  - *Genes, erbB-2
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods/*standards
MH  - Pathology, Clinical/methods/*standards
MH  - Reproducibility of Results
MH  - Tissue Array Analysis
MH  - United Kingdom
EDAT- 2010/05/13 06:00
MHDA- 2010/08/14 06:00
CRDT- 2010/05/13 06:00
PHST- 2010/05/13 06:00 [entrez]
PHST- 2010/05/13 06:00 [pubmed]
PHST- 2010/08/14 06:00 [medline]
AID - HIS3493 [pii]
AID - 10.1111/j.1365-2559.2010.03493.x [doi]
PST - ppublish
SO  - Histopathology. 2010 Feb;56(3):297-304. doi: 10.1111/j.1365-2559.2010.03493.x.