PMID- 20460120
OWN - NLM
STAT- MEDLINE
DCOM- 20101103
LR  - 20151119
IS  - 1873-2933 (Electronic)
IS  - 0009-9120 (Linking)
VI  - 43
IP  - 10-11
DP  - 2010 Jul
TI  - Identification of a complex between fibronectin and aggrecan G3 domain in 
      synovial fluid of patients with painful meniscal pathology.
PG  - 808-14
LID - 10.1016/j.clinbiochem.2010.04.069 [doi]
AB  - OBJECTIVES: We previously described a panel of four cytokines biomarkers in knee 
      synovial fluid for acute knee pain associated with meniscal pathology. The 
      cytokine biomarkers included interferon gamma (IFN-gamma), interleukin 6 (IL-6), 
      monocyte chemotactic protein 1 (MCP-1), and macrophage inflammatory protein-1 
      beta (MIP-1beta). Validation studies using other immunologic techniques confirmed 
      the presence of IL-6, MCP-1 and MIP-1beta, but not IFN-gamma. Therefore we sought 
      the identity of the IFN-gamma signal in synovial fluid. METHODS: Knee synovial 
      fluid was collected from patients with an acute, painful meniscal injury, as well 
      as asymptomatic volunteers. A combination of high-pressure chromatography, mass 
      spectrometry and immunological techniques were used to enrich and identify the 
      protein components representing the IFN-gamma signal. RESULTS: A protein complex 
      of fibronectin and the aggrecan G3 domain was identified in the synovial fluid of 
      patients with a meniscal tear and pain that was absent in asymptomatic controls. 
      This protein complex correlated to the IFN-gamma signal. A novel enzyme-linked 
      immunosorbent assay (ELISA) was developed to specifically identify the complex in 
      synovial fluid. CONCLUSIONS: We have identified a protein complex of fibronectin 
      and aggrecan G3 domain that is a candidate biomarker for pain associated with 
      meniscal injury.
CI  - Copyright 2010 The Canadian Society of Clinical Chemists. Published by Elsevier 
      Inc. All rights reserved.
FAU - Scuderi, Gaetano J
AU  - Scuderi GJ
AD  - Stanford University, Stanford, CA, USA.
FAU - Woolf, Naruewan
AU  - Woolf N
FAU - Dent, Kaitlyn
AU  - Dent K
FAU - Golish, S Raymond
AU  - Golish SR
FAU - Cuellar, Jason M
AU  - Cuellar JM
FAU - Cuellar, Vanessa G
AU  - Cuellar VG
FAU - Yeomans, David C
AU  - Yeomans DC
FAU - Carragee, Eugene J
AU  - Carragee EJ
FAU - Angst, Martin S
AU  - Angst MS
FAU - Bowser, Robert
AU  - Bowser R
FAU - Hanna, Lewis S
AU  - Hanna LS
LA  - eng
PT  - Journal Article
DEP - 20100511
PL  - United States
TA  - Clin Biochem
JT  - Clinical biochemistry
JID - 0133660
RN  - 0 (Aggrecans)
RN  - 0 (Biomarkers)
RN  - 0 (Fibronectins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aggrecans/*analysis/chemistry/*metabolism
MH  - Biomarkers/analysis
MH  - Chromatography, High Pressure Liquid
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fibronectins/*analysis/chemistry/*metabolism
MH  - Humans
MH  - Interferon-gamma/immunology
MH  - Mass Spectrometry
MH  - Menisci, Tibial/chemistry/*pathology
MH  - Prospective Studies
MH  - Protein Structure, Tertiary
MH  - Signal Transduction/immunology
MH  - Synovial Fluid/*chemistry
MH  - Young Adult
EDAT- 2010/05/13 06:00
MHDA- 2010/11/04 06:00
CRDT- 2010/05/13 06:00
PHST- 2010/02/10 00:00 [received]
PHST- 2010/04/06 00:00 [revised]
PHST- 2010/04/22 00:00 [accepted]
PHST- 2010/05/13 06:00 [entrez]
PHST- 2010/05/13 06:00 [pubmed]
PHST- 2010/11/04 06:00 [medline]
AID - S0009-9120(10)00202-X [pii]
AID - 10.1016/j.clinbiochem.2010.04.069 [doi]
PST - ppublish
SO  - Clin Biochem. 2010 Jul;43(10-11):808-14. doi: 10.1016/j.clinbiochem.2010.04.069. 
      Epub 2010 May 11.