PMID- 20460734
OWN - NLM
STAT- MEDLINE
DCOM- 20100907
LR  - 20100526
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 74
IP  - 5
DP  - 2010
TI  - Development of a method for the identification of S-IgA-coated bacterial 
      composition in mouse and human feces.
PG  - 968-73
AB  - Some commensal intestinal bacteria in humans and mice are coated with secretory 
      immunoglobulin A (S-IgA). It has been suggested that the S-IgA coating of 
      commensal bacteria does not occur at random and thus identification of 
      S-IgA-coated bacterial genera/species should help in elucidating the interaction 
      between S-IgA and commensal intestinal bacteria, but no method of identifying the 
      genera/species of S-IgA-coated bacteria has been established. To identify 
      S-IgA-coated bacterial composition, we developed a method combining 
      immunohistochemical detection of S-IgA and subsequent 16S rRNA targeted 
      fluorescence in situ hybridization (FISH) analysis. Human and mouse fecal S-IgA 
      coated bacterial composition was evaluated by this newly developed method with 10 
      frequently-used FISH probes. Fecal S-IgA-coated bacterial composition was 
      successfully analyzed by this method, and this suggests that Enterobacteriaceae 
      is preferably coated with S-IgA, whereas Bacteroides/Prevotella and 
      Lactobacillus/Enterococcus groups appear to be poorly coated with S-IgA.
FAU - Tsuruta, Takeshi
AU  - Tsuruta T
AD  - Laboratory of Nutritional Biochemistry, Department of Applied Bioscience, 
      Graduate School of Agriculture, Hokkaido University, Sapporo, Japan. 
      ahshoun@mail.ecc.u-tokyo.ac.jp
FAU - Inoue, Ryo
AU  - Inoue R
FAU - Iwanaga, Toshihiko
AU  - Iwanaga T
FAU - Hara, Hiroshi
AU  - Hara H
FAU - Yajima, Takaji
AU  - Yajima T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100507
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Immunoglobulin A, Secretory)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Bacteria/*classification/genetics/isolation & purification/*metabolism
MH  - Bacterial Typing Techniques/*methods
MH  - Base Sequence
MH  - Feces/*microbiology
MH  - Female
MH  - Glass
MH  - Humans
MH  - Immunoglobulin A, Secretory/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Reproducibility of Results
EDAT- 2010/05/13 06:00
MHDA- 2010/09/08 06:00
CRDT- 2010/05/13 06:00
PHST- 2010/05/13 06:00 [entrez]
PHST- 2010/05/13 06:00 [pubmed]
PHST- 2010/09/08 06:00 [medline]
AID - JST.JSTAGE/bbb/90801 [pii]
AID - 10.1271/bbb.90801 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2010;74(5):968-73. doi: 10.1271/bbb.90801. Epub 2010 
      May 7.