PMID- 20461403 OWN - NLM STAT- MEDLINE DCOM- 20100909 LR - 20240109 IS - 1432-2048 (Electronic) IS - 0032-0935 (Linking) VI - 232 IP - 2 DP - 2010 Jul TI - Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague. PG - 409-16 LID - 10.1007/s00425-010-1176-z [doi] AB - Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via Agrobacterium-mediated transformation, and putative transgenic lines were developed. The presence of the transgene in these putative transgenic lines was determined using polymerase chain reaction (PCR), and transgene integration and transgene copy number were confirmed following Southern blot analysis. The presence of specific F1-V transcripts was confirmed by reverse-transcriptase (RT)-PCR. Using monoclonal antibodies, ELISA and western blot analysis revealed that the expected antigenic F1-V protein was successfully expressed in transgenic lines. Mice immunized subcutaneously with lettuce expressing the F1-V antigen developed systemic humoral responses as 'proof of concept' of using lettuce as a production platform for the F1-V immunogen that could be used as a candidate plant-based vaccine against plague. FAU - Rosales-Mendoza, Sergio AU - Rosales-Mendoza S AD - Facultad de Ciencias Quimicas, Universidad Autonoma de San Luis Potosi, San Luis Potosi, Mexico. FAU - Soria-Guerra, Ruth E AU - Soria-Guerra RE FAU - Moreno-Fierros, Leticia AU - Moreno-Fierros L FAU - Alpuche-Solis, Angel G AU - Alpuche-Solis AG FAU - Martinez-Gonzalez, Luzmila AU - Martinez-Gonzalez L FAU - Korban, Schuyler S AU - Korban SS LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20100512 PL - Germany TA - Planta JT - Planta JID - 1250576 RN - 0 (Antigens, Bacterial) RN - 0 (LcrV protein, Yersinia) RN - 0 (Plague Vaccine) RN - 0 (Pore Forming Cytotoxic Proteins) RN - 0 (Recombinant Fusion Proteins) SB - IM MH - Animals MH - Antigens, Bacterial/genetics/*immunology/*metabolism MH - Blotting, Western MH - Enzyme-Linked Immunosorbent Assay MH - Lactuca/genetics/*metabolism MH - Mice MH - Mice, Inbred BALB C MH - Plague/*immunology/microbiology MH - Plague Vaccine/genetics/*immunology/*metabolism MH - Plants, Genetically Modified/genetics/metabolism MH - Polymerase Chain Reaction MH - Pore Forming Cytotoxic Proteins/genetics/*immunology/*metabolism MH - Recombinant Fusion Proteins/genetics/immunology/metabolism MH - Rhizobium/genetics MH - Yersinia pestis/*immunology/pathogenicity EDAT- 2010/05/13 06:00 MHDA- 2010/09/10 06:00 CRDT- 2010/05/13 06:00 PHST- 2010/01/20 00:00 [received] PHST- 2010/04/15 00:00 [accepted] PHST- 2010/05/13 06:00 [entrez] PHST- 2010/05/13 06:00 [pubmed] PHST- 2010/09/10 06:00 [medline] AID - 10.1007/s00425-010-1176-z [doi] PST - ppublish SO - Planta. 2010 Jul;232(2):409-16. doi: 10.1007/s00425-010-1176-z. Epub 2010 May 12.