PMID- 20461757
OWN - NLM
STAT- MEDLINE
DCOM- 20100909
LR  - 20100512
IS  - 1098-2264 (Electronic)
IS  - 1045-2257 (Linking)
VI  - 49
IP  - 7
DP  - 2010 Jul
TI  - High-resolution analysis of genomic alteration on chromosome arm 8p in urothelial 
      carcinoma.
PG  - 642-59
LID - 10.1002/gcc.20775 [doi]
AB  - Loss of chromosome arm 8p, sometimes in combination with amplification of 
      proximal 8p, is found in urothelial carcinoma (UC) and other epithelial cancers 
      and is associated with more advanced tumor stage. We carried out array 
      comparative genomic hybridization on 174 UC and 33 UC cell lines to examine 
      breakpoints and copy number. This was followed by a detailed analysis of the cell 
      lines using fluorescence in situ hybridization (FISH) and, in some cases, M-FISH, 
      to refine breakpoints and determine translocation partners, heterozygosity 
      analysis, and analysis of expression of selected genes. We showed an overall 
      pattern of 8p loss with reduced heterozygosity and reduced gene expression. 
      Amplification was seen in some samples and shown in the cell line JMSU1 to 
      correlate with overexpression of ZNF703, ERLIN2, PROSC, GPR124, and BRF2. Apart 
      from the centromere, no single breakpoint was overrepresented, and we postulate 
      that frequent complex changes without consistent breakpoints reflect the need for 
      alterations of combinations of genes. The region around 2 Mb, which was 
      homozygously deleted in one cell line and includes the gene ARHGEF10 and the 
      micro-RNA hsa-mir-596, is one candidate tumor suppressor gene region.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Williams, Sarah V
AU  - Williams SV
AD  - Leeds Institute of Molecular Medicine, St James's University Hospital, Leeds, UK.
FAU - Platt, Fiona M
AU  - Platt FM
FAU - Hurst, Carolyn D
AU  - Hurst CD
FAU - Aveyard, Jo S
AU  - Aveyard JS
FAU - Taylor, Claire F
AU  - Taylor CF
FAU - Pole, Jessica C M
AU  - Pole JC
FAU - Garcia, Maria J
AU  - Garcia MJ
FAU - Knowles, Margaret A
AU  - Knowles MA
LA  - eng
GR  - C6228/A5433/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Carcinoma, Transitional Cell/*genetics
MH  - Cell Line, Tumor
MH  - *Chromosome Aberrations
MH  - Chromosome Breakpoints
MH  - Chromosome Deletion
MH  - *Chromosomes, Human, Pair 8
MH  - DNA Copy Number Variations
MH  - Gene Amplification
MH  - Humans
MH  - Loss of Heterozygosity
MH  - Urinary Bladder Neoplasms/*genetics
EDAT- 2010/05/13 06:00
MHDA- 2010/09/10 06:00
CRDT- 2010/05/13 06:00
PHST- 2010/05/13 06:00 [entrez]
PHST- 2010/05/13 06:00 [pubmed]
PHST- 2010/09/10 06:00 [medline]
AID - 10.1002/gcc.20775 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2010 Jul;49(7):642-59. doi: 10.1002/gcc.20775.