PMID- 20462591
OWN - NLM
STAT- MEDLINE
DCOM- 20100922
LR  - 20131121
IS  - 1095-7103 (Electronic)
IS  - 0021-9797 (Linking)
VI  - 348
IP  - 1
DP  - 2010 Aug 1
TI  - A copper(II) thiosemicarbazone complex built on gold for the immobilization of 
      lipase and laccase.
PG  - 96-100
LID - 10.1016/j.jcis.2010.04.016 [doi]
AB  - A self-assembled monolayer (SAM) of imidazole-2-carbaldehyde thiosemicarbazone 
      (H(2)ImTSC) on gold was formed and characterized by ATR-FTIR, Time-of-Flight 
      Secondary Ion Mass Spectrometry (ToF-SIMS) and X-ray Photoelectron Spectroscopy 
      (XPS). The self-assembly of the ligand through its thioenolate group was 
      confirmed by ToF-SIMS and the presence of XPS peaks at 161.9 (S(2p1/2)) and 
      163.1eV (S(2p3/2)). The two nitrogen donor atoms of self-assembled HImTSC were 
      able to coordinate (kappa(2)-N,N) copper(II) when set to interact with a CuCl(2) 
      solution upon a second deprotonation of the ligand. This way, two types of 
      modified gold sheets for the immobilization of lipase and laccase were obtained: 
      (a) SAM of the ligand on gold (Au-HImTSC), and (b) SAM of HImTSC with a second 
      monolayer of copper(II) (Au-ImTSC-Cu(II)). The highest immobilization of enzyme 
      was achieved for laccase on Au-ImTSC-Cu(II) according to XPS and enzymatic 
      activity determinations. Copper(II) played a an important recognition role 
      through coordination to the enzyme and/or electrostatic interactions. 
      Nevertheless, the positively charged surface of Au-ImTSC-Cu(II) affected the 
      activity of laccase.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Rodriguez-Arguelles, M Carmen
AU  - Rodriguez-Arguelles MC
AD  - Departamento de Quimica Inorganica, Universidade de Vigo, 36310 Vigo, Spain.
FAU - Villalonga, Reynaldo
AU  - Villalonga R
FAU - Serra, Carmen
AU  - Serra C
FAU - Cao, Roberto
AU  - Cao R
FAU - Sanroman, M Angeles
AU  - Sanroman MA
FAU - Longo, Maria A
AU  - Longo MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100414
PL  - United States
TA  - J Colloid Interface Sci
JT  - Journal of colloid and interface science
JID - 0043125
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Thiosemicarbazones)
RN  - 7440-57-5 (Gold)
RN  - 789U1901C5 (Copper)
RN  - EC 1.10.3.2 (Laccase)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - Copper/*chemistry
MH  - *Enzymes, Immobilized
MH  - Gold/*chemistry
MH  - Laccase
MH  - Lipase
MH  - Materials Testing
MH  - Surface Properties
MH  - Thiosemicarbazones/*chemistry
EDAT- 2010/05/14 06:00
MHDA- 2010/09/24 06:00
CRDT- 2010/05/14 06:00
PHST- 2010/02/05 00:00 [received]
PHST- 2010/04/08 00:00 [revised]
PHST- 2010/04/09 00:00 [accepted]
PHST- 2010/05/14 06:00 [entrez]
PHST- 2010/05/14 06:00 [pubmed]
PHST- 2010/09/24 06:00 [medline]
AID - S0021-9797(10)00400-5 [pii]
AID - 10.1016/j.jcis.2010.04.016 [doi]
PST - ppublish
SO  - J Colloid Interface Sci. 2010 Aug 1;348(1):96-100. doi: 
      10.1016/j.jcis.2010.04.016. Epub 2010 Apr 14.