PMID- 20462636
OWN - NLM
STAT- MEDLINE
DCOM- 20100712
LR  - 20100623
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 47
IP  - 11-12
DP  - 2010 Jul
TI  - Differential activation and maturation of two porcine DC populations following 
      TLR ligand stimulation.
PG  - 2103-11
LID - 10.1016/j.molimm.2010.03.016 [doi]
AB  - Dendritic cells (DCs) are at the interface of innate and adaptive immune 
      responses. Once activated via triggering of their pattern recognition receptors 
      (PRRs), they acquire a mature state and migrate to the lymph nodes where they 
      activate T cells and direct the immune response. Compounds that trigger PRRs are 
      potential vaccine adjuvants, hence in this study we stimulated two porcine DC 
      populations, namely monocyte-derived DCs (MoDCs) and blood DCs (BDCs), with a 
      broad range of toll-like receptors (TLRs) ligands and assessed the 
      activation/maturation state of these porcine DCs. In order to determine if TLR 
      ligands would have an effect on porcine DCs, we characterized the expression of 
      TLRs and demonstrated that MoDCs and BDCs expressed the same set of TLRs but at 
      different levels. Of the TLR ligands examined, lipopolysaccharide (LPS) and poly 
      I:C were the most potent activators of MoDCs, inducing the up-regulation of 
      co-stimulatory molecules CD80/86 and the chemokine receptor CCR7, and production 
      of pro-inflammatory cytokines interleukin (IL)-6 and tumor necrosis factor 
      (TNF)alpha. The most effective in inducing BDCs activation were LPS and class A 
      CpG oligodeoxynucleotide (ODN), resulting in up-regulation of chemokine receptor 
      (CCR)7 and down-regulation of CCR2 and CCR5, production of IL-12p40, and 
      expression of a broad range of chemokines that were able to attract porcine 
      immune cells.
CI  - (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Auray, Gael
AU  - Auray G
AD  - Vaccine & Infectious Disease Organization, University of Saskatchewan, Saskatoon, 
      Canada.
FAU - Facci, Marina R
AU  - Facci MR
FAU - van Kessel, Jill
AU  - van Kessel J
FAU - Buchanan, Rachelle
AU  - Buchanan R
FAU - Babiuk, Lorne A
AU  - Babiuk LA
FAU - Gerdts, Volker
AU  - Gerdts V
LA  - eng
PT  - Journal Article
DEP - 20100511
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Cytokines)
RN  - 0 (Ligands)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*physiology
MH  - Ligands
MH  - Receptors, CCR2/analysis
MH  - Receptors, CCR5/analysis
MH  - Swine/*immunology
MH  - Toll-Like Receptors/*physiology
EDAT- 2010/05/14 06:00
MHDA- 2010/07/14 06:00
CRDT- 2010/05/14 06:00
PHST- 2010/01/29 00:00 [received]
PHST- 2010/03/19 00:00 [revised]
PHST- 2010/03/23 00:00 [accepted]
PHST- 2010/05/14 06:00 [entrez]
PHST- 2010/05/14 06:00 [pubmed]
PHST- 2010/07/14 06:00 [medline]
AID - S0161-5890(10)00110-0 [pii]
AID - 10.1016/j.molimm.2010.03.016 [doi]
PST - ppublish
SO  - Mol Immunol. 2010 Jul;47(11-12):2103-11. doi: 10.1016/j.molimm.2010.03.016. Epub 
      2010 May 11.