PMID- 20464435
OWN - NLM
STAT- MEDLINE
DCOM- 20110222
LR  - 20211203
IS  - 1438-2199 (Electronic)
IS  - 0939-4451 (Linking)
VI  - 39
IP  - 5
DP  - 2010 Nov
TI  - Evidence for cell cycle-dependent, rapamycin-resistant phosphorylation of 
      ribosomal protein S6 at S240/244.
PG  - 1487-92
LID - 10.1007/s00726-010-0615-2 [doi]
AB  - The ribosomal protein S6 is essential for the formation of the subunits of higher 
      eukaryotic ribosomes, and S6 heterozygosity leads to early embryonal lethality in 
      mice. S6 is phosphorylated at clustered residues S235/236 and S240/244 upon 
      numerous physiological and pathological stimuli. So far, the S6Kinases, S6K1 and 
      S6K2 are the only proven S6 S240/244 phosphorylating enzymes in mammalian cells. 
      The activity of these S6Kinases is strictly regulated via the mammalian target of 
      rapamycin (mTOR) enzyme complex with raptor, named mTORC1. In time course 
      experiments with the mTORC1 inhibitor rapamycin we here demonstrate 
      rapamycin-resistant phosphorylation of the ribosomal protein S6 at S240/244. 
      Serum-restimulation experiments further demonstrated that this 
      rapamycin-resistant S6 240/244 phosphorylation is induced via serum factors in a 
      cell cycle-dependent manner. Our data allow new insights into the regulation of 
      S6 phosphorylation and provide evidence for the existence of rapamycin-resistant 
      S6 phosphorylating kinase activities.
FAU - Rosner, M
AU  - Rosner M
AD  - Institute of Medical Genetics, Medical University of Vienna, Wahringer Strasse 
      10, 1090, Vienna, Austria.
FAU - Hengstschlager, M
AU  - Hengstschlager M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100513
PL  - Austria
TA  - Amino Acids
JT  - Amino acids
JID - 9200312
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Proteins)
RN  - 0 (Ribosomal Protein S6)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Cell Cycle
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Multiprotein Complexes
MH  - Phosphorylation
MH  - Proteins/antagonists & inhibitors/metabolism
MH  - Ribosomal Protein S6/*antagonists & inhibitors/metabolism
MH  - Sirolimus/*pharmacology
MH  - Structure-Activity Relationship
MH  - TOR Serine-Threonine Kinases
EDAT- 2010/05/14 06:00
MHDA- 2011/02/23 06:00
CRDT- 2010/05/14 06:00
PHST- 2010/04/26 00:00 [received]
PHST- 2010/04/27 00:00 [accepted]
PHST- 2010/05/14 06:00 [entrez]
PHST- 2010/05/14 06:00 [pubmed]
PHST- 2011/02/23 06:00 [medline]
AID - 10.1007/s00726-010-0615-2 [doi]
PST - ppublish
SO  - Amino Acids. 2010 Nov;39(5):1487-92. doi: 10.1007/s00726-010-0615-2. Epub 2010 
      May 13.