PMID- 20470941
OWN - NLM
STAT- MEDLINE
DCOM- 20100812
LR  - 20131121
IS  - 1873-5487 (Electronic)
IS  - 0188-4409 (Linking)
VI  - 41
IP  - 2
DP  - 2010 Feb
TI  - Detection of fusion genes in formalin-fixed paraffin-embedded tissue sections of 
      rhabdomyosarcoma by RT-PCR and fluorescence in situ hybridization in Mexican 
      patients.
PG  - 119-24
LID - 10.1016/j.arcmed.2010.02.005 [doi]
AB  - BACKGROUND AND AIMS: Rhabdomyosarcoma (RMS) is a pediatric tumor whose 
      classification is based on histological criteria according to two main subgroups, 
      embryonal RMS (ERMS) and alveolar RMS (ARMS). The majority but not all ARMS carry 
      the specific PAX3(7)/FKHR translocation. The type of translocation in patients 
      with ARMS defines the prognosis. METHODS: We retrospectively analyzed 30 cases of 
      ARMS in Mexican patients and evaluated the fusion status of the genes using 
      RT-PCR and fluorescence in situ hybridization (FISH) in formalin-fixed 
      paraffin-embedded tissues (FFPET). RESULTS: From 25 samples (83%) with optimal 
      RNA quality, RT-PCR revealed 15 cases (50%) with the t(2;13)/PAX3-FKHR. Only one 
      case (3%) was positive to t(1;13)/PAX7-FKHR and nine cases (30%) were 
      fusion-negative. Correspondingly, using FISH, the t(2;13)/PAX3-FKHR was found 
      positive in 19 cases (63.5%), one case (3%) revealed the t(1;13)/PAX7/FKHR and 
      ten cases (33.5%) were fusion-negative by this method. Five cases were not 
      evaluable by RT-PCR but recovered by FISH. Only four of the total revealed 
      t(2;13); the other was fusion-negative. CONCLUSIONS: FISH technique is more 
      sensitive when FFPET is used to describe the chromosomal translocation of ARMS. 
      These Latino patients showed an association of the t(2;13) in older patients 
      (mean: 9 years) and negative translocation in younger patients (mean: 4 years) (p 
      <0.05). Both t(2;13) and negative-fusion were present in patients with clinical 
      stages III and IV (p <0.05). There was a nonsignificant trend of t(2;13) to lower 
      overall survival than negative-fusion status.
CI  - Copyright 2010 IMSS. Published by Elsevier Inc. All rights reserved.
FAU - Eguia-Aguilar, Pilar
AU  - Eguia-Aguilar P
AD  - Departamento de Patologia, Hospital Infantil de Mexico Federico Gomez, Mexico, 
      D.F., Mexico.
FAU - Ponce-Castaneda, Veronica
AU  - Ponce-Castaneda V
FAU - Najera-Garcia, Nayelli
AU  - Najera-Garcia N
FAU - Nieto-Martinez, Karem
AU  - Nieto-Martinez K
FAU - Kofman-Alfaro, Susana
AU  - Kofman-Alfaro S
FAU - Sadowinski-Pine, Stanislaw
AU  - Sadowinski-Pine S
FAU - Valencia-Mayoral, Pedro
AU  - Valencia-Mayoral P
FAU - Arenas-Huertero, Francisco
AU  - Arenas-Huertero F
FAU - Perezpena-Diazconti, Mario
AU  - Perezpena-Diazconti M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Med Res
JT  - Archives of medical research
JID - 9312706
RN  - 0 (Recombinant Fusion Proteins)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Child
MH  - Formaldehyde
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Kaplan-Meier Estimate
MH  - Mexico
MH  - Paraffin Embedding
MH  - Recombinant Fusion Proteins/*genetics
MH  - Retrospective Studies
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Rhabdomyosarcoma/classification/*genetics/mortality/pathology
EDAT- 2010/05/18 06:00
MHDA- 2010/08/13 06:00
CRDT- 2010/05/18 06:00
PHST- 2009/10/30 00:00 [received]
PHST- 2010/01/25 00:00 [accepted]
PHST- 2010/05/18 06:00 [entrez]
PHST- 2010/05/18 06:00 [pubmed]
PHST- 2010/08/13 06:00 [medline]
AID - S0188-4409(10)00012-3 [pii]
AID - 10.1016/j.arcmed.2010.02.005 [doi]
PST - ppublish
SO  - Arch Med Res. 2010 Feb;41(2):119-24. doi: 10.1016/j.arcmed.2010.02.005.